The aim of the paper is to analyze instances of vegetalization, which is the X IS A PLANT metaphor, in John Henry Newman’s collection of sermons, published as Sermons on Subjects of the Day (1843). One group of metaphors are ontological metaphors, whose source domain is an entity (Lakoff, Johnson 2003[1980]). They can be classified as reifications, vegetalizations, animalizations, personifications, and deifications, which corresponds to the hierarchy of the so-called Great Chain of Being. As claimed by Krzeszowski (1997), these metaphors play an important role in expressing the axiological dimension of language, since they can express specific values of their target domains. In Christian discourse, vegetalizations contribute to the conceptualization of such notions from the religious sphere as God, grace, the Kingdom of God, the Christian life, the Church, or evil.
In this study, a SYBR Green-based real-time quantitative polymerase chain reaction (qPCR) assay was developed for rapid detection of porcine parvovirus (PPV) 6. Primer pairs targeting the conserved regions of PPV6 Capsid gene were designed. Sensitivity analyses revealed the lowest detection limit of the SYBR Green-based real-time PCR assay to be 47.8 copies/μL, which indicated it was 1000 times higher than that found in the conventional PCR investigations. This assay was specific and showed no cross-species amplification with other six porcine viruses. The assay demonstrated high repeatability and reproducibility; the intra- and inter-assay coefficients of variation were 0.79% and 0.42%, respectively. The positive detection rates of 180 clinical samples with SYBR Green-based real-time PCR and conventional PCR were 12.22% (22/180) and 4.44% (8/180), respectively. Our method is sensitive, specific, and reproducible. The use of SYBR Green-based real-time PCR may be suitable for the clinical detection and epidemiological investigation of PPV6.
Inspired by H. G. Wells' The Island of Doctor Moreau, M. Renard in his Le Docteur Lerne, sous-dieu (1908) portrays a mad scientist experimenting on living organisms, creating hybrids of humans, animals and plants. Renard extends the anachronistic theory of the Chain of Being to include machines, which he sees as the future of humanity, securing the place of his Lerne in the vanguard of transumanist literature. This paper aims to examine the novel’s intertextual references to both fictional and actual history of science.
The article presents a new optimization tool supporting supply chain management in the multi-criteria aspect. This tool was implemented in the EPLOS system (European Logistics Services Portal system). The EPLOS system is an integrated IT system supporting the process of creating a supply and distribution network in supply chains. This system consists of many modules e.g. optimization module which are responsible for data processing, generating results. The main objective of the research was to develop a system to determine the parameters of the supply chain, which affect its efficiency in the process of managing the goods flow between individual links in the chain. These parameters were taken into account in the mathematical model as decision variables in order to determine them in the optimization process. The assessment of supply chain management effectiveness was carried out on the basis of the global function of the criterion consisting of partial functions of the criteria described in the mathematical model. The starting point for the study was the assumption that the effectiveness of chain management is determined by two important decision-making problems that are important for managers in the supply chain management process, i.e. the problem of assigning vehicles to tasks and the problem of locating logistics facilities in the supply chain. In order to solve the problem, an innovative approach to the genetic algorithm was proposed, which was adapted to the developed mathematical model. The correctness of the genetic algorithm has been confirmed in the process of its verification.
Onion yellow dwarf virus (OYDV), an aphid-borne potyvirus is one of the major viral pathogens of garlic causing significant yield losses worldwide. It is found almost everywhere in the world where Allium species is grown. The aim of this study was to test the presence of OYDV infection in garlic from Ethiopia. The presence of the virus was tested by Reverse transcription polymerase chain reaction (RT-PCR). The direct sequencing of the PCR product produced a sequence of 296 bp. Sequence analysis showed 89.27% sequence homology with an isolate from Australia (HQ258894) and 89.29% with an isolate from Spain (JX429964). A phylogenetic tree constructed with MEGA 7.0 revealed high levels of homology with various isolates of OYDV from all over the world and thus further confirmed the identity of the virus.
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