Plant secondary metabolites have a variety of functions, including mediating relationships between organisms, responding to environmental challenges, and protecting plants against infections, pests, and herbivores. In a similar way, through controlling plant metabolism, plant microbiomes take part in many of the aforementioned processes indirectly or directly. Researchers have discovered that plants may affect their microbiome by secreting a variety of metabolites, and that the microbiome could likewise affect the metabolome of the host plant. Pesticides are agrochemicals that are employed to safeguard humans and plants from numerous illnesses in urban green zones, public health initiatives, and agricultural fields. The careless use of chemical pesticides is destroying our ecology. As a result, it is necessary to investigate environmentally benign alternatives to pathogen management, such as plant-based metabolites. According to literature, plant metabolites have been shown to have the ability to battle plant pathogens. Phenolics, flavonoids, and alkaloids are a few of the secondary metabolites of plants that have been covered in this study.

A method for obtaining of alkaloid preparations (A, fraction and pure alkaloid perchlorates ofA5 one) from bitter lupin (Lupinus albus L. cv. Bac) extract and evaluation of their usefulness for plant protection are described. The activity of the extract as well as its fraction A, and two major alkaloids of A3 one was tested on pests such as large cabbage white (Pieris brassicae L.) and small cabbage white (Pieris rapae L.) fed with leaves of cabbage treated with these preparations. Fractionation of the extract to obtain A, fraction and alkaloids perch I orates ofA3 one has been sufficient to eliminate impurities that may potentially cause undesirable by-effects in biological tests. Significant changes in various stages of development of insects were observed. Generally, fraction A, of lupin extract caused decrease in mass of fed feed as well as weight of objects observed. Furthermore, numerous visible damages of fed larvae and pupae that caused their inability to generate normal imagines (butterflies) were observed. Perchlorates of lupanine and 13-hydroxylupanine derived from lupin extract A3 fraction have not shown that effect, particularly on P. brassicae. Above supports an evidence that only natural bioconjugates of active substance may perform strong biological activity. In spite of similarity of tested insect species considerable differences between them in reaction to preparations were noticed.

This study aimed to evaluate the bioherbicidal effect of aqueous fresh extracts of leaves and roots of the Aloe vera plant on the broad leaf weed growth of Sonchus oleraceus associated faba bean plants. During the winters of 2020/2021 and 2021/2022, two pot experiments were carried out in the greenhouse of the National Research Center. Leaf and root aqueous extracts of Aloe vera were applied as soil and/or spray treatments at different concentrations. The results showed that there was significant inhibition in the fresh and dry biomass of S. oleraceus and was maximum with application of soil treatment (10%) of the leaf extract sequenced by spraying leaf extract at 20%. Furthermore, the inhibition of the weed growth was accompanied by an increase in the growth and yield of faba bean. The results indicated that phenols, flavonoids, alkaloids, tannins and saponins were present in the leaf extract, and there were smaller amounts of tannins and saponins in the root extract than in the leaf extract. Total phenols, flavonoids, alkaloids in the leaf extract was more than three times that of the root extract. The results also revealed that the presence of higher concentrations of natural substances in the leaf extract than in the root extracts gave it its efficiency in inhibiting the growth of S. oleraceus weeds.

We compared the biochemical profiles of Physalis ixocarpa hairy roots transformed with Agrobacterium rhizogenes ATCC and A4 strains with non-transformed root cultures. The studied clones of A4- and ATCC-induced hairy roots differed significantly; the latter showed greater growth potential and greater ability to produce secondary metabolites (tropane alkaloids) and to biotransform hydroquinone to arbutin. We compared glucose content, alanine and aspartate aminotransferase activity, and L-phenylalanine ammonia-lyase activity. We analyzed markers of prooxidant/antioxidant homeostasis: catalase, ascorbate peroxidase, oxidase, glutathione peroxidase and transferase activity, and the levels of ascorbate, glutathione, tocopherol and lipid peroxidation. We found that transformation induced strain-specific regulation, including regulation based on redox signals, determining the rate of allocation of carbon and nitrogen resources to secondary metabolism pathways. Our results provide evidence that A. rhizogenes strain-specific modification of primary metabolites contributed to regulation of secondary metabolism and could determine the ability of P. ixocarpa hairy root clones to produce tropane alkaloids and to convert exogenously applied hydroquinone to pharmaceutically valuable arbutin. Of the studied parameters, glucose content, L-phenylalanine ammonia-lyase activity and alanine aminotransferases activity may be indicators of the secondary metabolite-producing potential of different P. ixocarpa hairy root clones.

The purpose of this study was to investigate the effect of a biological humic preparation – “HUMIN PLUS”, made from natural raw materials – environmentally friendly lake sapropel on the biological development of agricultural crops. The study consisted in obtaining information and assessing the effect of a biological product on the dynamics of seedlings development, planting density, as well as crop productivity. To assess the preparation, as well as to identify the effect on the stages of plant ontogenesis, the physicochemical parameters were studied, including the content of humates, and trace elements in the sapropel extract. To interpret the effect of the growth regulator on the seed germination energy, an adaptive-neural inference system was used. To establish the nature of the action of preparation on the development biology of plant, in the conditions of Kazakhstan, a series of experiments were carried out at different stages of ontogenesis of agricultural crops. It was found that the action of the “HUMIN PLUS” preparation significantly increases the content of essential and nonessential amino acids. The findings have established that the sapropel extract “HUMIN PLUS” affects the biological activity of plants, accelerating the seed germination and increasing the productivity of agricultural crops in Kazakhstan.