Fatigue cracks in machine components are subjected to stress fields induced by the external load and residual stresses resulting from the surface treatment. Stress fields in such cases are characterized by non-uniform distributions and handbook stress intensity factor solutions for such configurations are not available. The method presented below is based on the generalized weight function technique enabling the stress intensity factors to be calculated for any Mode I loading applied to arbitrary planar convex crack. The method is particularly suitable for modeling fatigue crack growth in presence of complex stress fields.

Cell culture transplantation is very promising in the treatment of various diseases. Cells obtained from a number of sources have been analysed to provide a basis for further studies in the area of regenerative medicine. The objective of the study was to compare morphological and phenotypic changes in cat adipose tissue and bone marrow cell cultures from the first to fifth passages. Adipose tissue and bone marrow were used to obtain cell cultures (coming from 3 cats) using standard methods with own modification. Phenotype changes were monitored by CD-marker identification and CD pan-keratin. The cytogenetic analysis was performed on 50 metaphase plates of cell cultures from the first to fifth passage. Cytogenetic assays showed that the adipose tissue cell culture (ATCC) at all passages was more stable than the bone marrow cell culture (BMCC).