TY - JOUR N2 - This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fluorescens SBW25 and Burkholderia xenovorans LB400 species were dominant. L1 - http://www.czasopisma.pan.pl/Content/85936/PDF/10265-Volume40-Issue2-paper_09.pdf L2 - http://www.czasopisma.pan.pl/Content/85936 PY - 2014 IS - No 2 DO - 10.2478/aep-2014-0020 KW - mycorrhiza KW - helper bacteria (MHB) KW - 16sRNA gene A1 - Ząbkiewicz, Anna A1 - Myga-Nowak, Magdalena A1 - Bandurska, Katarzyna A1 - Paczyńska, Justyna A1 - Szybecka, Anna A1 - Krupa, Piotr PB - Polish Academy of Sciences VL - vol. 40 DA - 2014 T1 - The Application of PCR Reaction for Identification of MHB Bacteria Species UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/85936 T2 - Archives of Environmental Protection ER -