The paper expounds relevant results of some of the present author’s experi- ments defining the strapdown IMU sensors’ errors and their propagation into and within DGPS/IMU. In order to deal with this problem, the author conducted both the laboratory and field-based experiments. In the landborne laboratory the stand-alone Low-Cost IMU MotionPak MKII was verified in terms of the accelerometer bias, scale factor, gyroscope rotation parameters and internal temperature cross-correlations. The waterborne field-trials based on board dedicated research ships at the lake and at the busy small sea harbour were augmented by the landborne ones. These experiments conducted during the small, average, and high dynamics of movement provided comparative sole- GPS, stand-alone DGPS and integrated DGPS/IMU solution error analysis in terms of the accuracy and the smoothness of the solution. This error estimation was also carried on in the context of the purposely-erroneous incipient DGPS/IMU initialisation and alignment and further in the circumstances of on-flight alignment improvement in the absence of the signal outages. Moreover, the lake-waterborne tests conducted during extremely low dynamics of movement informed about the deterioration of the correctly initialised DGPS/IMU solution with reference to the stand-alone DGPS solution and sole- GPS solution. The above-mentioned field experiments have checked positively the DGPS /MKI research integrating software prepared during the Polish/German European Union Research Project and modified during the subsequent Project supported by the Polish Committee for Scientific Research.

The DiSTFA method (Displacements and Strains using Transformation and Free Adjustment) was presented in Kamiński (2009). The method has been developed for the determination of displacements and strains of engineering objects in unstable reference systems, as well as for examining the stability of reference points. The DiSTFAG (Gross errors) method presented in the paper is the extension of the DiSTFA method making it robust to gross errors. Theoretical considerations have been supplemented with an example of a practical application on a simulated 3D surveying network.

The method that is proposed in the present paper is a special case of squared M split estimation. It concerns a direct estimation of the shift between the parameters of the functional models of geodetic observations. The shift in question may result from, for example, deformation of a geodetic network or other non-random disturbances that may influence coordinates of the network points. The paper also presents the example where such shift is identified with a phase displacement of a wave. The shift is estimated on the basis of wave observations and without any knowledge where such displacement took place. The estimates of the shift that are proposed in the paper are named Shift- M split estimators.

The paper presents the results of research on the possibilities of fixing ship position coordinates based on results of surveying bearings on navigational marks with the use of the CCD camera. Accuracy of the determination of ship position coordinates, expressed in terms of the mean error, was assumed to be the basic criterion of this estimation. The first part of the paper describes the method of the determination of the resolution and the mean error of the angle measurement, taken with a camera, and also the method of the determination of the mean error of position coordinates when two or more bearings were measured. There have been defined three software applications assigned for the development of navigational sea charts with accuracy areas mapped on. The second part contains the results of studying accuracy in fixing ship position coordinates, carried out in the Gulf of Gdansk, with the use of bearings taken obtained with the Rolleiflex and Sony cameras. The results are presented in a form of diagrams of the mean error of angle measurement, also in the form of navigational charts with accuracy fields mapped on. In the final part, basing on results obtained, the applicability of CCD cameras in automation of coastal navigation performance process is discussed.

The work presents the results of studies on dependence of effectiveness of chosen robust estimation methods from the internal reliability level of a geodetic network. The studies use computer-simulated observation systems, so it was possible to analyse many variants differing from each other in a planned way. Four methods of robust estimation have been chosen for the studies, differing substantially in the approach to weight modifications. For comparative reasons, the effectiveness studies have also been conducted for the very popular method in surveying practice, of gross error detection basing on LS estimation results, the so called iterative data snooping. The studies show that there is a relation between the level of network internal reliability and the effectiveness of robust estimation methods. In most cases, in which the observation contaminated by a gross error was characterized by a low index of internal reliability, the robust estimation led to results being essentially far from expectations.

In deformation analyses, it is important to find a stable reference frame and therefore the stability of the possible reference points must be controlled. There are several methods to test such stability. The paper’s objective is to examine one of such methods, namely the method based on application of R-estimation, for its sensitivity to gross errors. The method in question applies three robust estimators, however, it is not robust itself. The robustness of the method depends on the number of unstable points (the fewer unstable points there are, the more robust is the proposed method). Such property makes it important to know how the estimates applied and the strategy itself respond to a gross error. The empirical influence functions (EIF) can provide necessary information and help to understand the response of the strategy for a gross error. The paper presents examples of EIFs of the estimates, their application in the strategy and describes how important and useful is such knowledge in practice.

In the recent years three-dimensional buildings modelling based on an raw air- borne laser scanning point clouds, became an important issue. A significant step towards 3D modelling is buildings segmentation in laser scanning data. For this purpose an algorithm, based on the multi-resolution analysis in wavelet domain, is proposed in the paper. The proposed method concentrates only on buildings, which have to be segmented. All other objects and terrain surface have to be removed. The algorithm works on gridded data. The wavelet-based segmentation proceeds in the following main steps: wavelet decomposition up to appropriately chosen level, thresholding on the chosen and adjacent levels, removal of all coefficients in the so-called influence pyramid and wavelet reconstruction. If buildings on several scaling spaces have to be segmented, the procedure should be applied iteratively. The wavelet approach makes the procedure very fast. However, the limitation of the proposed procedure is its scale-based distinction between objects to be segmented and the rest.

A new method to transform from Cartesian to geodetic coordinates is presented. It is based on the solution of a system of nonlinear equations with respect to the coordinates of the point projected onto the ellipsoid along the normal. Newton’s method and a modification of Newton’s method were applied to give third-order convergence. The method developed was compared to some well known iterative techniques. All methods were tested on three ellipsoidal height ranges: namely, (-10 – 10 km) (terrestrial), (20 – 1000 km), and (1000 – 36000 km) (satellite). One iteration of the presented method, implemented with the third-order convergence modified Newton’s method, is necessary to obtain a satisfactory level of accuracy for the geodetic latitude ( σ φ < 0.0004”) and height ( σ h < 10 − 6 km, i.e. less than a millimetre) for all the heights tested. The method is slightly slower than the method of Fukushima (2006) and Fukushima’s (1999) fast implementation of Bowring’s (1976) method.

The paper deals with large-scale crustal deformation due to hydrological surface loads and its influence on seasonal variation of GPS estimated heights. The research was concentrated on the area of Poland. The deformation caused by continental water storage has been computed on the basis of WaterGAP Hydrological Model data by applying convolution of water masses with appropriate Green’s function. Obtained site displacements were compared with height changes estimated from GPS observations using the Precise Point Positioning (PPP) method. Long time series of the solutions for 4 stations were used for evaluation of surface loading phenomena. Good agreement both in amplitude and phase was found, however some discrepancies remain which are assigned to single point positioning technique deficiencies. Annual repeatability of water cycle and demanding procedure for computing site displacements for each site, allowed to develop a simple model for Poland which could be applied to remove (or highly reduce) seasonal hydrological signal from time series of GPS solutions.

The green peach aphid, Myzus persicae (Sulzer), is a polyphagous and holocyclic aphid which significantly damages agricultural crops. In the current study, the effects of micronutrients on some secondary metabolites of bell pepper (Capsicum annum L.) leaves and their subsequent influence on the life table parameters of M. persicae were investigated under greenhouse conditions. The flavonoid content in bell pepper leaves significantly changed following micronutrient treatments in the wavelength of 270 nm while there were no significant differences in the wavelengths 300 and 330 nm. The highest anthocyanin content was recorded after Fe treatment (3.811 mg ⋅ ml–1) while the total phenolic content in the bell pepper leaves increased after Mn (541.2 mg ⋅ ml–1) treatment compared to Fe (254.5 mg ⋅ ml–1) and control (216.33 mg ⋅ ml–1), respectively. The highest values of intrinsic (r) and finite rates of population increase (λ) of M. persicae were gained with Zn (0.320 and 1.377 day–1, respectively) treatment although the highest and the lowest values of the mean generation time (T) were found with Fe and Zn (14.07 and 12.63 days, respectively) treatments, respectively. Our findings suggest that Mn, more than Zn micronutrients, decreased ecological fitness of green peach aphid and may help enhance the efficiency of pest control techniques.

Soybean [Glycine max (L.)], one of the most important crops in Argentina, is commonly infected by Colletotrichum truncatum, the causal agent of anthracnose. Tagetes filifolia essential oil (EO) is presented as a natural approach to minimize the dose of chemical fungicides applied to the crop. The fungus Trichoderma harzianum is used as a biocontrol agent because of its ability to produce secondary metabolites that destroy cell walls of phytopathogenic fungi. However, its performance can be affected when it is exposed to chemical fungicides. The objective of this work was to evaluate the antifungal activity of T. filifolia EO both individually and combined with chemical fungicides against C. truncatum, and its effect on T. harzianum. Fungi were isolated from soybean crops. The following pesticides were assessed: carbendazim (F1), difenoconazole (F2) and trifloxystrobin + cyproconazole (F3). The EO was obtained from native plants and its chemical composition was analyzed by gas chromatography–mass spectrometry (GC–MS). The minimum fungicide concentration (MFC) was determined for each compound. Fungicides were combined with the EO to look for combinations that allowed a reduction of pesticide doses. Among fungicides, F1 showed the strongest antifungal activity against C. truncatum (MFC = 0.25 μl ⋅ l–1) and T. harzianum (MFC = 1.5 μl ⋅ l–1). The sensitivity of both fungi to the EO was lower than to fungicides. The EO presented MFCs of 6,000 and 9,000 μl ⋅ l–1 against C. truncatum and T. harzianum. The EO and F1 affected the growth of T. harzianum at concentrations that controlled C. truncatum (31 and 10%). Eight combinations of fungicides and the EO allowed fungicide concentration reductions of up to 80%, although the growth of the biocontrol strain was also affected. The results demonstrated that T. filifolia EO can be used to control anthracnose and reduce doses of chemical fungicides applied to soybean crops. Its effect on T. harzianum should be considered in the design of integrated pest management strategies.

Repellent usage against rodents is almost not provided anymore. Permission to use many rodent repellent substances under European Union (EU) plant protection regulations has not been renewed in recent years. Some approval for chemical substances have not been renewed due to their toxicological properties, and for some biorational approvals have also not been renewed due to lack of financial support together with other concerns. Some other rodent repellent substances possessing accurate properties in a secondary way have also been withdrawn. Thus, the use of almost ten active substances is now illegal. The lack of support and the resultant orphan use may be explained by the relatively small market and possible business together with the expectations of modern substance application requirements. As a result, the opportunity to consider new biorational substances as candidates is therefore open. Plant based food substances are preferred candidates for plant protection considering their favourable toxicological characteristics. Capsicum oleoresin, a mixture obtained from two spice species (Capsicum annuum and C. frutescens), is one of them with appropriate repellent properties. An application under EU Plant Protection Product regulation has recently been submitted and may become a new repellent for biological control agent against seed predators.

Wheat grain discoloration, a worldwide disease that lowers grain quality and decreases grain yield, does not have a single etiology. It has been proposed that it is a consequence of an abiotic mechanism, a response to environmental conditions or enzymatic activity. It has also been suggest that it is a biotic mechanism, a fungal infection principally by Alternaria spp. and Bipolaris sorokiniana. The present work was carried out to analyze the possible etiology of this disease in nine durum wheat genotypes from two localities of southern Buenos Aires province (Argentina) on two sowing dates. Incidence (percentage of grain discoloration) was recorded and mycobiota associated with this pathology was registered following ISTA rules. Peroxidase activity in an extract obtained from grains belonging to genotypes of the locality that showed the highest incidence was measured.

The incidence among genotypes, localities and sowing dates varied, although the genotypes with the higher and lower values of incidence were the same for all the variables tested. The fungus Alternaria spp. was isolated the most frequently followed by Fusarium spp., while Bipolaris sorokiniana was found the least frequently. Peroxidase activity showed that all the treatments had similar levels of enzymatic activity, but there was no clear differentiation between controls either between genotypes with the lowest or the highest incidence values. This suggests that peroxidase activity did not have a clear relationship with grain discoloration. In this research, it is presumed that fungal infection is the main cause of this disease.

Stem canker and black scurf of potato (Solanum tuberosum L.) caused by Rhizoctonia solani Kühn are important and epidemic diseases in potato-growing regions worldwide, including Iran. In this study, 120 isolates were retrieved from infected stem canker from six potato- growing regions in Iran (Isfahan, Ardebil, Fars, Hamedan, Kurdestan and Kerman). Out of these, 30 isolates were selected as representatives for genetic and virulence analysis. The isolates were analyzed by one sequence analyzes of the ITS-rDNA region, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), as well as virulence studies. Based on sequence analysis of the ITS-rDNA region, all 30 isolates were assigned to the anastomosis group (AG) and all were assigned to AG-3 PT. Cluster analysis using the unweighted pair group method with the arithmetic averages (UPGMA) method for both RAPD and ISSR markers revealed that they were divided into three main groups, with no correlation to geographical regions of the isolates. Pathogenicity tests showed that all isolates were pathogenic on potato cv. Agria; however, virulence variability was observed among the isolates. The grouping based on RAPD analysis and virulence variability was not correlated.

Ditylenchus destructor is a serious pest of numerous economically important plants worldwide. The population of this nematode species was isolated from the root zone of Ammophila arenaria on a Baltic Sea sand dune. This population’s morphological and morphometrical characteristics corresponded to D. destructor data provided so far, except for the stylet knobs’ height (2.1–2.9 vs 1.3–1.8) and their arrangement (laterally vs slightly posteriorly sloping), the length of a hyaline part on the tail end (0.8–1.8 vs 1–2.9), the pharyngeal gland arrangement in relation to the intestine (dorsal or ventral vs dorsal, ventral or lateral) and the appearance of vulval lips (smooth vs annulated). Ribosomal DNA sequence analysis confirmed the identity of D. destructor from a coastal dune.

This study focused on the effect of heavy metal cobalt ions (at concentrations of 1–1000 ppm) on the development and enzymatic activity of four entomopathogenic fungi: Beauveria bassiana, Beauveria brongniartii, Isaria fumosorosea and Metarhizium robertsii, commonly used in biological plant protection. It was found that each of the tested species of fungi reacted individually to contact with the Co2+ ions at their various concentrations. Depending on the variants of the experiment carried out, there were changes in the development of the mycelia (mainly growth inhibition) and their morphological features (color and structure) in comparison to the control samples. Co2+ ions had a fungistatic effect on all fungal strains, whereas a fungicidal effect was noted at concentrations of 750 ppm and 1000 ppm against M. robertsii and I. fumosorosea, respectively. In addition, there was a discrepancy in enzymatic activity between the tested fungal species developing in the medium with varying concentrations of metal salt. The inhibitory effect of Co2+ ions on lipase production was observed in I. fumosorosea. Protease production was stimulated in B. bassiana at all Co2+ concentrations, whereas in M. robertsii this effect was noted at 1 ppm. The changing dynamics of extracellular fungal hydrolases, due to the action of Co2+ ions, may translate into the role of these microorganisms in the processes of insect pathogenesis. This work suggests that severe pollution of the environment by cobalt could be a restrictive factor for the development and pathogenicity of entomopathogenic fungi and must be taken into account for their successful application in biological plant protection.

Septoria melissae Desm., the most important pathogen of lemon balm (Melissa officinalis) occurs each year on plantations. The fungus may cause serious yield losses in the absence of proper plant protection. Breeding resistant or tolerant cultivars could play an important role in plant protection of medicinal plants. However, only a few descriptions of tolerant varieties of lemon balm are available. The goal of this work was to evaluate the susceptibility of three accessions of M. officinalis against the pathogen of Septoria leaf spot under field conditions at Budapest-Soroksár (Hungary) in 2017–2018. Differences in susceptibility of the accessions were observed in both years. The accession of M. officinalis subsp. altissima proved to be the least susceptible to Septoria infection. The frequency of the infected leaves was only 5.1 and 28.1% in 2017 and 2018, respectively. However, the cultivar M. officinalis subsp. officinalis ‘Lorelei’ turned out to be the most susceptible to the pathogen with an average infection level of 26.1 and 66.6%, 1.3–6.1 times higher than that of the other accessions in each year, respectively. Development of disease tolerant M. officinalis cultivars may be an effective tool in the plant protection of lemon balm.

Onion yellow dwarf virus (OYDV), an aphid-borne potyvirus is one of the major viral pathogens of garlic causing significant yield losses worldwide. It is found almost everywhere in the world where Allium species is grown. The aim of this study was to test the presence of OYDV infection in garlic from Ethiopia. The presence of the virus was tested by Reverse transcription polymerase chain reaction (RT-PCR). The direct sequencing of the PCR product produced a sequence of 296 bp. Sequence analysis showed 89.27% sequence homology with an isolate from Australia (HQ258894) and 89.29% with an isolate from Spain (JX429964). A phylogenetic tree constructed with MEGA 7.0 revealed high levels of homology with various isolates of OYDV from all over the world and thus further confirmed the identity of the virus.

The study was conducted at the University of Nebraska Pesticide Application and Technology Laboratory in North Platte, Nebraska in July 2015. Two application volume rates (100 and 200 l · ha−1) and three nozzle types (XR, AIXR, TTI) were selected at two flow rates (0.8 and 1.6 l · min−1) and at a single application speed of 7.7 km · h−1. Each collector type [Mylar washed (MW), Mylar image analysis (MIA), water-sensitive paper (WSP), and Kromekote (KK)] was arranged in a randomized complete block design. Each nozzle treatment was replicated twice, providing six cards of each collector type for each nozzle treatment. A water + 0.4% v/v Rhodamine WT spray solution was applied, given the fluorescent and visible qualities of Rhodamine, which allows it to be applied over all the collector types. MW had the highest coverage at 18.3% across nozzle type, followed by WSP at 18%, KK at 12% and lastly by MIA at 4%. MW resulted in a 58% increase in coverage, WSP in a 56% increase, and KK only an increase of 39% when the volume rate was doubled from 100 l · ha−1 to 200 l · ha−1 across nozzle type. MW coverage was similar to KK for half of the nozzles (XR 11002, XR 11004, AIXR 11002). Droplet number density fixed effects were all significant for nozzle type and collector type (p < 0.001) as was the interaction of nozzle type and collector type (p < 0.001). Results from this study suggest a strong correlation to data produced with WSP and MW collectors, as there was full agreement between both types except for the TTI 11004. Using both collector types in the same study would allow for a visual understanding of the distribution of the spray, while also giving an idea of the concentration of that distribution.

The present study was conducted to evaluate the insecticidal efficiency and safety of zinc oxide nanoparticles (ZnO NPs) and hydrophilic silica nanoparticles (SiO2 NPs) against: adults of rice weevil (Sitophilus oryzae L.); red flour beetle (Tribolium castaneum Herbst.) and cowpea beetle (Callosobruchus maculatus F.) results showed that, both ZnO NPs and hydrophilic SiO2 NPs exhibited a significant toxic effect (df, F and p < 0.5) against S. oryzae and C. maculatus at the highest concentration while T. castaneum showed high resistance against the two tested materials. At the end of the experiment, recorded mortality was: 81.6, 98.3 and 58.3% at the highest concentration used for each insect (0.3, 2 and 8 gm ⋅ kg–1 of SNPs with C. maculatus, S. oryzae and T. castaneum, respectively), while mortality was 88.3, 100 and 38.3% at the highest concentration used for each insect (0.6, 2.5 and 8 gm ⋅ kg–1 of ZnO NPs with C. maculatus, S. oryzae and T. castaneum, respectively). Both tested materials caused high reductions in F1-progeny (%) with C. maculatus and S. oryzae. Histopathological examination of male mice livers showed hepatic architecture with congested blood sinusoids, binucleated hepatocytes nuclei, dilated central vein and margainated chromatin in some nuclei. Histopathological assessment of the lungs showed normal histoarchitecture. There were no differences in alveolar septa, bronchiolar and epithelium of the treated and untreated animals. Silica and zinc oxide nanoparticles have a good potential to be used as stored seed protectant alternatives if applied with proper safety precautions.

The main open-field producer regions of cucurbits (watermelon, squash, melon and cucumber) in Panama (Los Santos, Herrera and Coclé provinces) were surveyed for molecular identification, occurrence and distribution of Thrips palmi (the most important pest thrip species on cucurbits in Panama), Frankliniella intonsa and Frankliniella cephalica during the growing seasons of 2009 to 2013 and 2017 to 2018. Forty plots were surveyed and DNA extracts of 186 thrips (larvae and adults) were analyzed by multiplex PCR, using a set of T. palmi-specific primers in combination with a set of insect-universal primers. DNA extracts corresponding to 174 individual thrips (93.5%) rendered both PCR products of expected size with T. palmi-specific and insect-universal primers, whereas the remaining DNA extracts corresponding to 12 individual thrips (6.5%) only rendered the product of the expected size with insect-universal primers. Sequencing of those PCR products and BLAST analysis allowed for the identification of F. intonsa and F. cephalica. Thrips palmi was detected in all three provinces, while F. intonsa and F. cephalica were detected in Herrera and Los Santos provinces. To our knowledge, this is not only the first detection of F. intonsa in Panama, but also the first detection of F. cephalica in Panamanian cucurbit crops.

The rose flea beetle, RFB (Luperomorpha xanthodera Fairmaire 1888) is a new flower pest in Europe. In 2012, it was brought accidentally to central Poland. To search for this introduced species in the area adjacent to the site of the first finding, 29 plant species belonging to five botanical families (Lamiaceae, Brassicaceae, Asteraceae, Plantaginaceae, Crassulaceae) were monitored over a 3-year-long study (2016−2018). RFB were found on 11 herbaceous/ ornamental plant species (Lamiaceae, Brassicaceae, Asteraceae) along with feeding damage to the flowers. White mustard (Sinapis alba L., Brassicaceae), hyssop (L.), and Monarda spp. (Lamiaceae) were its most preferred host plants. In each season, RFB females preferred host plants which bloomed abundantly and vividly. However, among the examined plant species there was a large variation in the year-to-year RFB abundance. Over the examined period the RFB extended its abundance exponentially, and its population survived and established itself in the area. The general sex ratio of the beetles was strongly female biased. In the female pool, females with conspicuously swollen abdomens predominated. The results of our study provide more insight into RFB behaviour, its establishment and spreading into new areas. To support the evidence for the RFB risk factor as an agricultural/ horticultural pest, further research should focus on the beetles’ biology, reproductive tactics, larval host plant preference, larva-inflicted damage and harmfulness, the impact of the RFB on the native fauna, as well as its further local and distant migration propensity. Presently our knowledge about these aspects is still fragmentary.

Weeds are one of the most important limiting factors in the production of chickpea (Cicer arietinum) in Iran, especially in autumn sown chickpea. Weed density and biomass in autumn chickpea are seven and two and a half times higher than the spring chickpea, respectively. The weed damage to chickpea in Tabriz, Kermanshah and West Azerbaijan was estimated at 48.3, 57 and 36%, respectively. Sixty-four weed species were identified in chickpea fields. Convolvulus arvensis L. and Galium tricornutum Dandy have the highest presence in chickpea fields. Pyridate and linuron are the only herbicides registered for use in chickpea fields in Iran. However, research results show that fomesafen and isoxaflutole are the most appropriate herbicides for chickpea fields. Oxyfluorfen, imazethapyr, metribuzin, trifluralin, simazine, terbutryn and pendimethalin are the major herbicides studied in weed control research. The combination of herbicides and mechanical control is one of the effective methods to reduce weeds. Hand weeding and cultivation between rows are the most effective mechanical methods of weed control. High nitrogen enhances weed dry weight. Safflower and barley residues reduce weed populations and biomass. Barley-chickpea and wheat-chickpea intercropping systems increase chickpea yield together with proper weed control. In future research, more attention should be paid to surfactants to reduce the use of herbicides, rotation crops and integrated weed management in chickpea.

Bread wheat is a major food crop on a global scale. Stripe rust, caused by Puccinia striiformis f. sp. tritici, has become one of the largest biotic stresses and limitations for wheat production in the 21st century. Post 2000 races of the pathogen are more virulent and able to overcome the defense of previously resistant cultivars. Despite the availability of effective fungicides, genetic resistance is the most economical, effective, and environmentally friendly way to control the disease. There are two major types of resistance to stripe rust: all-stage seedling resistance (ASR) and adult-plant resistance (APR). Although both resistance types have negative and positive attributes, ASR generally is race-specific and frequently is defeated by new races, while APR has been shown to be race non-specific and durable over time. Finding genes with high levels of APR has been a major goal for wheat improvement over the past few decades. Recent advancements in molecular mapping and sequencing technologies provide a valuable framework for the discovery and validation of new sources of resistance. Here we report the discovery of a precise molecular marker for a highly durable type of APR – high-temperature adult-plant (HTAP) resistance locus in the wheat cultivar Louise. Using a Louise × Penawawa mapping population, coupled with data from survey sequences of the wheat genome, linkage mapping, and synteny analysis techniques, we developed an amplified polymorphic sequence (CAPS) marker LPHTAP2B on the short arm of wheat chromosome 2B, which cosegregates with the resistant phenotype. LPHTAP2B accounted for 62 and 58% of phenotypic variance of disease severity and infection type data, respectively. Although cloning of the LPHTAP2B region is needed to further understand its role in durable resistance, this marker will greatly facilitate incorporation of the HTAP gene into new wheat cultivars with durable resistance to stripe rust.