Based on the analysis of a number of studies, it was found that to assess the state of the environment (including surface waters and soils) it is advisable to use indicators of microbiological pollution, which in general integrally reflect the state of the ecosystem. To assess the dynamics of changes in the pollution of the studied areas, a comparison of monitoring data with the corresponding level of pollution in protected areas (Vyzhnytsia National Nature Park) was used. Research methods included soil and surface water sampling, inoculation on appropriate nutrient selective media, counting of colony forming units (CFU) and other microbiological indicators. To assess the biological activity of soils, urease activity was determined by a method generally accepted in biochemistry. It is established that within the protected areas, despite some existing annual fluctuations, the relative stability of the studied indicators of the hydrosphere is preserved. Studies have shown that soils of anthropogenically altered landscapes are characterised by a high content of sanitary-indicative bacteria. As our research shows, according to the colony forming units (CFU), total microbial count, and titer of Escherichia coli, the soils selected in the protected area of the Vyzhnytsia National Nature Park correspond to the “pure” level. The soils of the territories out of the National Nature Park are characterised by high biological capacity, as evidenced by the level of activity of the enzyme urease and the ratio of the main forms of nitrogen compounds.

Mezyn National Natural Park is in the north part of the Korop District of the Chernihiv Region, 310 km2 in area, is characteristic for its strongly dismembered landscape that stipulates presence of varied localities, among them there are exterminated glaciofluvial plains, slopes and super streamside terraces. Aim of research included an expose of the value of the Mezyn NNP in maintenance of forest diversity of vascular plants and forest communities. In the Mezyn NNP there are 772 species of spontaneous flora, 194 species of the cultivated flora and 18 rarity species. The rarest species are concentrated in the centre and the east, prats of the park richer in vegetation cover. In the forest group of the Mezyn NNP 10 species of vascular plants are recordered in the Red book of the Ukraine. The wooden territory of the Mezyn NNP presents 38% and provides the variety of ecotopic terms after they provides a wide spectrum of representation and protection of forest species of vascular plants and corresponding phytocommunites.