The aim of the study was the determination the effect of foliar application of growth regulator containing Ti (Tytanit®), on Lolium multiflorum morphometry, photosynthetic activity, chlorophyll content and chemical composition of dry matter. A pot experiment was carried out in a plant breeding room of Siedlce University, Poland, in 2019. The experimental units were as follows: I) control – plants sprayed with distilled water; II) plants sprayed with 0.02% Tytanit concentration; III) plants sprayed with 0.04% Tytanit concentration; IV) plants sprayed with 0.06% Tytanit concentration. The following parameters were determined: the shoots number, the number and the length of leaf blades, the length of roots, the dry weight of roots, the dry weight of plants per pot and the content of chlorophyll a and b in leaf blades. In addition, maximum and actual efficiency of the leaf photosystem, photochemical and non- photochemical quenching coefficients and the content of total protein, crude fibre, monosaccharides, crude fat, crude ash, Ca, Mg, P, K and the ratio of K/(Ca + Mg), and Ca/P in the dry matter of plants were determined. Used in controlled conditions, the regulator contributed to the growth of most morphological characteristics, improved photosynthetic activity, increased the concentration of chlorophyll a and b, and