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Number of results: 14
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Abstract

The photo-oxidability of lipids taken from 32 samples of krill from different dates of catch has been examined for photooxidation. Relations were indicated between the rate of accumulation of peroxides in the process of lipids, exposure and content of lipids in krill, its iodine value and amount number of carotenoids.

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Authors and Affiliations

Anna Kołakowska
ORCID: ORCID
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Abstract

A composition of lipids of some Antarctic Crustacea (Euphausia superba. E. triacantha. Thysanoessa macrura and Mysidacea gen. sp. indet.) caught in the Admiralty Bay (South Shetlands) was compared. Lipids of E. superba differed in low content of waxes that evidences for different management of lipids than in other examined Crustacea.

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Authors and Affiliations

Anna Kołakowska
ORCID: ORCID
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Abstract

Using a thin layer chromatography the content and composition of krill lipids was examined in different sex and maturity stages. The content of lipids decreased in the following sequence: immature males — females with eggs — juvenile specimens — spent females — mature males. In females the differences concerned mainly phospholipids and waxes, in males — triacylglicerols; this fact proves the different utilisation of lipids for reproduction in both sexes.

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Authors and Affiliations

Anna Kołakowska
ORCID: ORCID
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Abstract

The amount and composition of lipids m some Antarctic animals were studied. The material consisted of crustaceans (Euphausia superba, Paramoera sp., Orchomene sp.), tunicates (Salpa thompsoni) and vertebrates (Notothenia rossi marmorata and Hydrurga leptonyx). The author's data are discussed on the background of available literature information.

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Authors and Affiliations

Anna Kołakowska
ORCID: ORCID
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Abstract

The production of biofuels using wastewater as a microalgae culture medium is a little explored technology, but with potential for success. In order to contribute to the knowledge of these technologies and their technical feasibility for microalgae growth, in this work the Chlorella sp. strain was cultivated in two types of effluents generated in an experimental farm located in eastern Colombia, before and after a biological treatment process. The consumption of the main nutrients that regulate growth and lipid production was evaluated, in order to extract, quantify, characterize and convert them into biodiesel. The results showed that Chlorella sp. growth and lipid production is more favourable in R2 medium of treated water than in R1 medium of raw water, mainly due to phosphorus limitation and higher N-NO3 concentration in R2 compared to R1. In the R2 medium culture, a percentage of 42.54% of long-chain fatty acids was found, which is necessary to obtain a high quality biodiesel. Finally, the best transesterification experiment allowed reaching a fatty acid methyl esters (FAME) percentage of 90.1 ± 2.7%. In general, the results demonstrated the potential viability of using the wastewater generated in the San Pablo farm to produce biomass with lipid content to obtain biodiesel, finding that where the concentration of nutrients, mainly nitrogen, has a great influence on the microalgal metabolism for lipid accumulation.
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Authors and Affiliations

Nestor Andres Urbina-Suarez
1
ORCID: ORCID
Andres Fernando Barajas-Solano
1
ORCID: ORCID
Janet Bibiana Garcia-Martinez
1
ORCID: ORCID
German Luciano Lopez-Barrera
1
ORCID: ORCID
Angel Dario Gonzalez-Delgado
2
ORCID: ORCID

  1. Francisco de Paula Santander University, Cúcuta, Colombia
  2. University of Cartagena, Avenida del Consulado Calle 30 No. 48-152, Cartagena, Bolívar, Colombia
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Abstract

Studies on the surface-active agents added during the cultivation process proved their effect on the synthesized bioproducts. The biosynthesis and accumulation of exopolysaccharide and biomass by Antarctic producer Cryptococcus laurentii AL65 were studied in submerged cultivation using different carbon sources in the culture medium added to a one-percent concentration (pentoses, hexoses and oligosaccharides). Sucrose was chosen as the most appropriate carbon source for the cell growth and extracellular polymer biosynthesis. Absorption of sucrose by a strain producer was monitored and the residual sugars were traced in the dynamics of the fermentation process. The effect of polyoxyethylene sorbitan monooleate (Tween 80) on the cell growth and the accumulation of the synthesized biopolymer were studied for the first time with the Antarctic producer. The optimal increase in the amount of accumulated exopolysaccharide was recorded at a concentration of 6% Tween 80 at 24 h from the start of fermentation. The surfactant treatment contributed to an increase in the total amount of lipids in the cell, to the increased biosynthesis of unsaturated fatty acids compared to saturated ones, and to the increased cellular synthesis of valuable metabolites such as phosphatidylinositol, phosphatidylserine and phosphatidylethanolamine. The impact of Tween 80 on intracellular and extracellular metabolites is a valuable asset in a possible scale-up of the process in industrial settings.
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Authors and Affiliations

Snezhana Rusinova-Videva
1
ORCID: ORCID
Zhana Petkova
2
ORCID: ORCID
Ginka Antova
2
ORCID: ORCID
Plamen Stoyanov
3 4
ORCID: ORCID
Tsvetelina Mladenova
3
ORCID: ORCID
Tzenka Radoukova
3
ORCID: ORCID

  1. The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria
  2. Department of Chemical Technology, University of Plovdiv Paisii Hilendarski, 24 Tz. Assen, 4000 Plovdiv, Bulgaria
  3. Department of Botany, University of Plovdiv Paisii Hilendarski, 24 Tz. Assen, 4000 Plovdiv, Bulgaria
  4. Department of Pharmacognosy and Pharmaceutical Chemistry, Medical University of Plovdiv, 15A V. Aprilov, 4000, Plovdiv, Bulgaria
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Abstract

Studies in human medicine have shown that in addition to affecting the reproductive system, the hormone estrogen also has cardioprotective effects. The present study hypothesized that ovariohysterectomized (OVH) dogs would have a higher incidence of cardiac dysfunction and impairment of lipid profiles compared to intact female dogs. Thirty healthy female dogs were divided into two groups, 15 intact female dogs and 15 OVH dogs. All the dogs underwent a physical examination, including investigation of physical parameters, blood collection for lipid profile measurement, thoracic radiography, electrocardiography and echocardiography. Physical examination parameters, electrocardiographic parameters, heart size and cardiac function in OVH dogs were not different when compared to intact female dogs. However, in the OVH dogs, triglyceride and very-low-density lipoprotein levels were increased, while high-density lipoprotein was significantly decreased compared to the intact female dogs (P<0.05). Differences between the groups in total cholesterol and low-density lipoprotein did not reach statistical signi- ficance. We concluded that estrogen deprivation in dogs can induce lipid profile impairment but not cardiac performance impairment 1 year after an ovariohysterectomy.

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Authors and Affiliations

C. Boonyapakorn
V. Punyapornwithaya
G. Sawatphakdee
N. Poolsawat
W. Pongkan
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Abstract

Entomopathogenic nematodes (EPNs) are promising as biocontrol agents for the most economically important insect pest attacking a wide range of host plants. Therefore, the aim of this work was to study the impact of four artificial diets and one natural food on numbers, weights, and total lipid content of the greater wax moth larvae, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) as well as the impact of these diets on the ability of nematode species Heterorhabditis bacteriophora and Steinernema carpocapsae to infect insects and multiply inside an insect host which had been reared on one of five different diets (D1, D2, D3, D4 and D5). The correlation between larvae weight and total lipid content, pathogenicity or multiplication of nematodes was also studied. The obtained results indicated that D2, D5 and D3 gave the highest growth or weights of larvae. The larvae produced weighed 3.71, 3.67 and 3.25 g from 50 g media, respectively. Statistically, larvae weights had a positive and significant correlation with the lipid content in larvae where r = 0.732. On the other hand, infective juveniles (IJs) of nematodes produced from insect hosts reared on D2 and D5 revealed more pathogenicity on larvae, since they caused the highest percent of mortality, 53.33 and 50.0% for H. bacteriophora, and 56.67 and 53.33% for S. carpocapsae, respectively. The total lipid content had a positive and highly significant correlation with the pathogenicity of the two nematode species where r = 0.97 and 0.971, respectively. Ultimately, the supplied foods of the artificial diets D2, D3 and natural beeswax (D5) gave the most suitable chance for developing insect growth and increasing the EPN quality and enhancing the potential of EPNs as biological control agents against different insect pests.
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Authors and Affiliations

Mona Ahmed Hussein
1
ORCID: ORCID
Hamdy Abdelnaby Salem
1
Sayed Hala
1
Salah Mahmoud
1

  1. Pests and Plant Protection Department, Agricultural and Biological Research Institute, National Research Center, Cairo, Egypt
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Abstract

The purpose of this study was to validate the applicability of specialized microbial consortium for the degradation of lipids in wastewater. An experimental model of the process is proposed that enables prediction of the required batch length. This model can be used for supervision of the process and to control cycles of the batch reactor. The study involved 4 reactors with microbial consortium obtained by inoculation from a commercially available biopreparate. Each reactor was fed a different load of lipid containing substrate. The biodiversity, settling characteristics and COD reductions were measured. The biodiversity of the microbial consortium changed within a range of ±15% depending on lipids concentration, as shown by the Shannon index and increasing amount of β-proteobacteria. Higher concentrations of lipids increased the biodiversity suggesting higher growth of microorganisms capable of utilizing lipids as energy and carbon source by producing lipid hydrolyzing enzymes. High lipid concentrations degrade the settling capabilities of the biomass. Higher lipid concentrations (0.5–2.0 [g/l]) increase the final COD (1445–2160 [mg O2/l]). The time necessary for substrate degradation changes with the initial concentration and can be predicted using the proposed model. The study showed that specialized microbial consortium is capable of reducing the lipids containing substrate and maintains its biodiversity suggesting that utilization of such consortia in multiple cycles of a batch reactor is possible. Future research should concentrate on assessing the biodiversity and effectiveness of substrate reduction after an increased number of batch reactor cycles.

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Authors and Affiliations

Witold Nocoń
1
ORCID: ORCID
Anna Węgrzyn
1
Mieczysław Metzger
1

  1. Silesian University of Technology, Gliwice, Poland
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Abstract

The aim of the present study was to determine changes in oxidative status in plasma of sows during periparturient period using markers of lipid and protein peroxidation intensity. Plasma from 12 pregnant gilts collected on days 14 and 1 before parturition and on days 1, 7 and 14 postpartum was used in the study. As indicators of alterations in the oxidative status, plasma concentrations of thiobarbituric acid reactive substances (TBARS) and sulphydryl groups (SH groups) were measured spectrophotometrically, as well as plasma concentrations of bityrosine and formylokinurenine were determined by the spectrofluorimetric method. Compared to the 14th day antepartum, the concentration of TBARS increased significantly on day 1 postpartum and then decreased significantly on days 7 and 14 after parturition. The concentration of SH groups increased significantly on the last day before parturition and decreased significantly on day 14 postpartum. The concentration of bityrosine increased significantly on the last day before parturition and decreased significantly on days 7 and 14 postpartum. The concentration of formylokinurenine increased significantly on the last day before parturition, remained at this level on the first day after parturition and then decreased slightly on days 7 and 14 postpartum. These results indicate that during periparturient period in sows, especially around the parturition, the oxidative/antioxidative balance is disturbed, which leads to oxidative stress. Considering that oxidative stress is potentially harmful for mothers and neonates, this may be an important factor in the increased risk of diseases occurring during this period.

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Authors and Affiliations

M. Szczubiał
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Abstract

The main cause of sperm chromatin damage is oxidative stress related to embryo development failure and adult infertility in mammals and also avian. Oxidative stress results in lipid peroxidation (LPO) causing cell damage. Lipid peroxidation is the oxidation of polyunsaturated fatty acids (PUFAs) in biological systems and causes changes in the physical structure and characteristics of the cell membrane. Due to the high amounts of PUFAs in the avian sperm membrane, its sperm seem susceptible to pe-roxidative damage and is a substantial factor in the fertilization capacity of sperm. The most commonly used methods for measuring LPO or its by-products, such as malondialdehyde (MDA) and 4-hydroksy-2-nonenal (4-HNE), in bird semen are based on the colorimetric method TBARS (thiobarbituric acid reactive substances) and on the use of a fluorescence probe (CC 11-BODIPY 581/591) as a marker to evaluate membrane lipid peroxidation. This review aims first to introduce LPO in avian semen and its effects on avian sperm and second to summarize the commonly applied methods of evaluating LPO and its damage in fresh and stored avian semen.
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Authors and Affiliations

A. Partyka
1
A. Babapour
2
M. Mikita
1
S. Adeniran
3
W. Niżański
1

  1. Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50-366 Wroclaw, Poland
  2. University of Tabriz, Faculty of Veterinary Medicine, Department of Basic Sciences, Tabriz, Iran
  3. Mountain Top University, College of Basic and Applied Sciences, Department of Biological Sciences, Ogun State, Nigeria
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Abstract

The aim of this study was to investigate the effect of the addition of fungal solid-state fermented product (FP) enriched with gamma-linolenic acid (GLA) and β-carotene to feed on the haematological and immunological parameters of broiler chickens. Eighty 1-day-old COBB 500 broiler chickens were divided into two groups. The control group was fed with basic diets and chickens of the experimental group received 10% addition of FP, while the amount of basic diet was reduced. FP was produced during a solid-state fermentation (SSF) process using Umbellopsis isabellina CCF2412 as a producer of GLA and β-carotene. After 38 days of feeding, blood samples were collected and analyzed. Lower total and LDL-cholesterol values were measured in blood samples of the experimental animals (p<0.05). However, the triacylglycerol content was higher in the experimental group (p<0.05). Significantly higher levels of hematocrit and hemoglobin, and lower eosinophil and basophil content in the experimental group were recorded (p<0.05). The experimental group showed higher numbers of B lymphocytes and greater phagocytic capacity (p<0.05). The results indicate that a fermented product produced by SSF, using the fungal strain Umbellopsis isabellina, is a good source of GLA and β-carotene, which can influence the biochemical, hematological and immunological parameters of broiler chickens.

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Authors and Affiliations

M. Bartkovský
D. Mudroňová
D. Marcinčáková
T. Klempová
E. Sesztáková
I. Maskaľová
V. Karaffová
I. Jaďuttová
M. Čertík
M. Hudák
S. Marcinčák
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Abstract

The aim of this paper is to show the basic principles of the anaerobic digestion process. All the stages of degradation, such as hydrolysis, acidogenesis, acetogenesis and methanogenesis are characterized. Biodegradable organic matter consists of three main types of substances: carbohydrates, proteins and lipids; the metabolic pathways of their decomposition are described. The last part of the paper presents the co-digestion process, its benefits and technological parameters required to make that process attractive from an economical and environmental point of view.
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Authors and Affiliations

Agnieszka Montusiewicz
Magdalena Lebiocka
Małgorzata Pawłowska
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Abstract

Background: Cardiovascular diseases are the first cause of death globally. Hypercholester-olemia is the most important factor responsible for atherosclerotic plaque formation and increasing cardiovascular risk. Reduction of LDL-C level is the most relevant goal for reduction of cardiovascular risk.
Aims: Real life adherence to guidelines concerning statin therapy in one center study population. Methods: We analyzed data collected in the Department of Internal Diseases from September 2019 to February 2020, obtained from 238 patients hospitalized in this time period. We assessed application of the new 2019 ESC/EAS Guidelines for the Management of Dyslipidaemias in daily clinical practice and compared effectiveness of LLT according to 2016 and 2019 guidelines.
Results: Only 1 in 5 patients with dyslipideamia achieve the 2019 ESC/EAS guideline-recommended levels of LDL-C with relation to their TCVR. We noticed that 20 of patients who did not achieve proper 2019 LDL level, meet the therapy targets established in year 2016. We observed that higher patient TCVR resulted in better compliance with guidelines and ordination of proper LLT. Most patients were on monotherapy with statins.
Conclusions: It could be beneficial to start treatment with double or even triple therapy especially in group with the highest LDL-C levels.
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Authors and Affiliations

Patrycja Cecha
1
Anna Chromik
1
Ilona Piotrowska
1
Michał Zabojszcz
1
Magdalena Dolecka-Ślusarczyk
1
Zbigniew Siudak
1

  1. Collegium Medicum, Jan Kochanowski University, Kielce, Poland

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