Petroleum pollution is still one or crucial environmental problems. Bioaugmcntation is a popular technique used in soil remediation. The aim of soil inoculation is acceleration or decomposition processes or improving the degradation efficiency. Effectiveness of bioaugmentation processes depends on the number and activity of microorganisms adapted to pollutant degradation. Enhancement of microorganisms' activity can be reached by the use or plants. Roots or plants excrete organic substances that stimulate microorganisms' growth. Among different species or plants interesting arc papilionaceous plants because of their nitrogen fixation ability in symbiosis with bacteria. The effects or using papilionaceous plants (Trifolium pratense), multiplied autochthonous microorganisms and commercial biopreparation in aged-petroleumpolluted soil were studied. The samples of soil were taken from the refinery in Czechowice-Dziedzice (Poland) and classified as heavily degraded with a C/N-ratio or 100:0.7. Investigations were conducted for 14 weeks. Microbiological analysis included: total bacteria, fungi, Actinomycetes and Pscudonionas counts. Concentration or heavy fractions, TPH (total petroleum hydrocarbons) and PAHs (polycyclic aromatic hydrocarbons) were measured at the start and at the end of the experiment. Presence of pap il ionaceous plant (Trifolium pratensei enhanced the growth or microorganisms, nitrogen concentration and biodegradation processes (removal of 63% or TPH, 44% or heavy fractions, 9% or 4-6 aromatic PAHand 80% of 2-3 aromatic PAH) in polluted soil. An increasing number of Pseudomonas species was observed in samples in which pollution removal was more effective.

The aim of this work was to determine the influence of native and alien keratinolytic fungi inocula on the petroleum hydrocarbon removal rate in soil covered and not covered with hair. The hair was the major nutrient for keratinolytic fungi and other soil microorganisms. The fungal inocula accelerated the petroleum hydrocarbon biodegradation process during the first month of the experiment. During the second month, TPH removal rates were similar for soil inoculated and not inoculated with fungi. The highest petroleum hydrocarbon removal rate was observed in soil inoculated with native fungal strains. The TPI-I removal rates were about 64% in soil covered with hair and 77% in soil not covered with hair. The lowest removal rate was observed in soil not inoculated with fungi (60%). The hair applied as additional nitrogen, sulfur and carbon source did not impact, or slightly inhibited, the petroleum hydrocarbon biodegradation process. The fungal inocula caused dramatic changes in soil fungal qualitative composition.