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Abstract

FA discharged from the wastewater treatment plant were extracted from purifi ed effl uents for the quantitative

and qualitative analysis. The treated sewage from municipal treatment plants was acidifi ed to pH <2 and extracted

with ion exchange resins in a laboratory column. After desorption with NH4

OH, the fulvic acids were condensed

under vacuum and tested for mass performance, UV-VIS light spectra, IR absorption spectra, elementary composition

and other elements. Their structure was analysed and compared to FA present in surface waters and in sewage treated

in other sewage treatment plants based on the authors’ own research and the literature data. The concentration of

FA in the treated sewage was 5.2 mg/L. There is a high interdependence between the IR spectrum analysis in the

visible light and the elementary composition of FA extracted from different environments, confi rming the conclusions

pertaining to the structure and properties of the acids being tested. The longer sewage is subjected to a biological

treatment process, the greater the degree of aromatic condensation and humus maturity of the FA contained within it.

FA contained in the sewage treated in the three biological sewage treatment plants have the ratio A2

/A3

(the ratio of the

absorbance of light with the wavelength of 250 and 300 nm) equal to the value 1.7. There is a high interdependence

between the IR spectrum analysis in the visible light and the elementary composition of FA extracted from different

environments, confi rming the conclusions pertaining to the structure and properties of the acids being tested.

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Authors and Affiliations

Anna Maria Anielak
Małgorzata Kryłów
Dominika Łomińska-Płatek
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Abstract

Soils of Russian European North were investigated in terms of stability and quality of organic matter as well as in terms of soils organic matter elemental composi-tion. Therefore, soil humic acids (HAs), extracted from soils of different natural zones of Russian North-East were studied to characterize the degree of soil organic matter stabilization along a zonal gradient. HAs were extracted from soil of different zonal environments of the Komi Republic: south, middle and north taiga as well as south tundra. Data on elemental composition of humic acids and fulvic acids (FAs) extracted from different soil types were obtained to assess humus formation mechanisms in the soils of taiga and tundra of the European North-East of Russia. The specificity of HAs elemental composition are discussed in relation to environmental conditions. The higher moisture degree of taiga soils results in the higher H/C ratio in humic substances. This reflects the reduced microbiologic activity in Albeluvisols sods and subsequent conser-vation of carbohydrate and amino acid fragments in HAs. HAs of tundra soils, shows the H/C values decreasing within the depth of the soils, which reflects increasing of aromatic compounds in HA structure of mineral soil horizons. FAs were more oxidized and contains less carbon while compared with the HAs. Humic acids, extracted from soil of different polar and boreal environments differ in terms of elemental composition winch reflects the climatic and hydrological regimes of humification.
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Authors and Affiliations

Evgeny Abakumov
Evgeny Lodygin
Vasily Beznosikov
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Abstract

The leachate problem is important and difficult to solve in Poland and in the world. The composition and their properties leachates depend on the age of the landfill, type of waste, climatic conditions and the mode of operation of the landfill. A significant part of landfilled waste is subject to so-called humification. This process stabilizes organic substances in the landfill and creates humic substances that penetrate into the leachate. The leaks contain many toxic impurities, such as PAHs, pesticides, polychlorinated biphenyls and other substances hazardous to human health and life, which can be sorbed by humic substances. Leachates from three municipal landfills, differing in the characteristics of the stored waste, were studied. Fulvic acids (FAs) were extracted on the basis of affinity for specific solvents along with the use of sorption. The obtained acids were subjected to a qualitative analysis of the content of micro-impurities, essential elements forming the structure of the fulvic acid molecule, and their infrared spectra were tested. It has been noticed that with the age of waste deposited, the content of elemental carbon increases, and the amount of oxygen and hydrogen decreases. The degree of purity of fulvic acids was influenced by the time of waste storage, and the sulfur content depended on their characteristics. With the time of waste storage, the characteristics of the acids obtained were approaching humic acids, and the intensity of absorption bands clearly increased. The spectra obtained correlate well with those of fulvic acids available in the literature, and the findings provide scientific confirmation of the need for further research on the characteristics of fulvic acids.
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Authors and Affiliations

Tomasz Orliński
1
Anna M. Anielak
1

  1. Department of Environmental Engineering, Institute of Water Supply and Environmental Protection, Cracow University of Technology, Poland
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Abstract

This study examined the process of filtering of infiltrated water containing excessive amounts or iron, manganese, and fulvic acids through two filtration beds-sand and zeolite-exhibiting catalytic properties. The fulvic acids that were added to the filtered water were extracted from mud in Kołobrzeg. The zeolite bed was modified with manganese oxide using our own technology and required periodic regeneration using 0.3% KMnO, solution. Our study showed the fulvic acids' negative effect on the process of water purification. The zeolite bed reduces this effect and is more effective than the quartz sand bed.
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Authors and Affiliations

Anna M. Anielak
Mariusz Wojnicz
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Abstract

Humic substances are polydisperse mixtures of structurally complex matters with different molecular weights. The complexity of molecular composition of humic substances is reflected through their physical and chemical properties and results in diverse interactions both with inorganic components and living organisms. The correlation of the molecular composition of humic and fulvic acids and their molecular weight distribution were analyzed by means of CP/MAS 13C NMR spectroscopy and size exclusion chromatography. Humic acids are a dynamic system containing macromolecular, oligomeric and low-molecular components. Fulvic acids are a monodisperse mixture of relatively low-molecular (up to 2 kDa) organic compounds. A significant correlation between the content of high and medium weight molecular fractions with labile fragments and low molecular weight fractions with hydrophobous fragments of humic acids has been revealed.

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Authors and Affiliations

Evgeny Lodygin
Roman Vasilevich
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Abstract

Data on the molecular structure of humic substances (HSs) of zonal soils for the southern, middle, northern taiga and southern tundra of northeastern European Russia have been obtained. This was accomplished using solid-state 13C nuclear magnetic resonance (13C NMR) technique. The soils under study vary in the point of genesis and degree of hydromorphism. The impact of environmental factors (temperature and humidity) on qualitative and quantitative composition of humic (HAs) and fulvic acids (FAs) has been determined. Excess moisture significantly affects HS accumulation and HS molecular structure: hydromorphic taiga soils accumulate HSs enriched by unoxidized aliphatic fragments, tundra soils – the ones enriched by carbohydrate fragments. Various conditions of soil genesis predefine the specific character of structural and functional parameters of HSs in the southern taiga to southern tundra soils, as is expressed through the increased portion of labile carbohydrate and amino acid fragments and methoxyl groups within the structure of HSs. The tundra humification is characterized by levelling-off of structural and functional parameters of major classes of specific organic compounds of soils – HAs and FAs.

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Authors and Affiliations

Evgeny Lodygin
Roman Vasilevich
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Abstract

Humification plays an important role in stabilization of organic matter in soils of the cryolithic zone. In this context, the degree of organic matter stabilization has been assessed, using instrumental methods, for permafrost peat soils of the eastern European Arctic, based on selected plots from within the Komi Republic (Russian Federation). Humic substances (HSs) isolated from the mire permafrost peats of the forest-tundra subzone of the European Arctic have been characterized in terms of molecular composition. This was accomplished using elemental and amino acid fragments (AAFs) composition. Solid-state 13C nuclear magnetic resonance (13C NMR) spectroscopy was utilized to identify the structure of HSs. Changes in the molar x(H) : x(C) ratio, ratio of aromatic to paraffin fragments and ratio of hydroxy AAFs to heterocyclic AAFs along the peat profiles have been revealed. They are due to the activation of cryogenic processes in the upper part of the seasonally thawing layer, the natural selection of condensed humic molecules, the botanical composition and degree of degradation of peat, which reflect the climatic features of the area in the Holocene. Humic acids and fulvic acids of the peat soils showed the prevalence of compounds with a low degree of condensation and a low portion of aromatic fragments. The aromaticity degree showed the trend to increase within the depth. Changes of quantitative and qualitative parameters of specific organic compounds occur at the permafrost boundary of peatlands, which can serve as an indicator of recent climate changes in environments from the high latitudes. The presented data can be useful in the evaluation of soil organic matter stabilization degree in the active layer and below the permafrost table.

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Authors and Affiliations

Roman Vasilevich
Evgeny Lodygin
Evgeny Abakumov
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Abstract

The purpose of this study was to investigate the effect of a biological humic preparation – “HUMIN PLUS”, made from natural raw materials – environmentally friendly lake sapropel on the biological development of agricultural crops. The study consisted in obtaining information and assessing the effect of a biological product on the dynamics of seedlings development, planting density, as well as crop productivity. To assess the preparation, as well as to identify the effect on the stages of plant ontogenesis, the physicochemical parameters were studied, including the content of humates, and trace elements in the sapropel extract. To interpret the effect of the growth regulator on the seed germination energy, an adaptive-neural inference system was used. To establish the nature of the action of preparation on the development biology of plant, in the conditions of Kazakhstan, a series of experiments were carried out at different stages of ontogenesis of agricultural crops. It was found that the action of the “HUMIN PLUS” preparation significantly increases the content of essential and nonessential amino acids. The findings have established that the sapropel extract “HUMIN PLUS” affects the biological activity of plants, accelerating the seed germination and increasing the productivity of agricultural crops in Kazakhstan.
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Authors and Affiliations

Raikhan J. Abutova
1
ORCID: ORCID
Marat K. Kozhakhmetov
1
ORCID: ORCID

  1. Kazakh National Agrarian Research University, Department of Technology and Safety of Food Products, 050010, 8 Abai Ave., Almaty, Republic of Kazakhstan

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