This study aimed to analyse the effect of anthropogenic activities on the spatial distribution of total nitrogen (TN) and total phosphate (TP) in Lake Maninjau, Indonesia, during the dry season. Sampling was carried out at ten observation locations representative for various activities around the lake. Cluster analysis and ANOVA were used to classify pollutant sources and observe differences between TN and TP at each site. Concentrations of TN and TP are categorised as oligotrophic-eutrophic. The ANOVA showed spatially that some sampling locations, such as the Tanjung Sani River, floating net cages, and hydropower areas have different TN concentrations. At the same time, TP levels were consistently significantly different across sampling sites. ANOVA and cluster analysis confirmed that floating net cages were the first cluster and the primary contributor to TN and TP. The second and third clusters come from anthropogenic activities around the lake, such as agriculture, settlement, and livestock. The fourth cluster with the lowest TN and TP is the river that receives the anthropogenic activity load but has a high flow velocity. The cluster change analysis needs to be conducted when there are future changes in the composition of floating net cages, agriculture, and settlements.