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Abstract

As the impact of global climate change increases, the interaction of biotic and abiotic stresses increasingly threatens current agricultural practices. The most effective solution to the problem of climate change and a decrease in the amount of atmospheric precipitation is planting extremely drought-resistant and high-yielding crops. Sorghum can grow in harsh conditions such as salinity, drought and limited nutrients, also it is an important part of the diet in many countries. Sorghum can be introduced in many zones of Kazakhstan. Plant height and yield of green plant biomass of 16 sorghum samples in arid conditions were determined based on a set of agrobiological characteristics for field screening. The height of the studied samples of grain sorghum was 0.47 ±0.03 m, and the height of sweet sorghum was much longer, reaching up to 2.88 ±0.12 m. Also, there was a strong difference in green biomass in cultivated areas under different soil and climatic conditions, the green biomass of sweet sorghum was 3.0 Mg∙ha –1, and in grain sorghum, it reached up to 57.4 Mg∙ha –1. Based on the data of the field assessment for various soil and climatic conditions, the following samples were identified for introduction into production: samples of sweet sorghum for irrigated and rainfed lands of the Almaty Region and in the conditions of non-irrigation agriculture of the Aktobe Region – a promising line ICSV 93046. For non-irrigation agriculture of the Akmola Region, genotypes of sweet and grain sorghum are ‘Chaika’, ‘Kinelskoe 4’ and ‘Volzhskoe 44’.
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Authors and Affiliations

Rauan Zhapayev
1
Kristina Toderich
2 3
Gulya Kunypiyaeva
1
Meruyert Kurmanbayeva
4
Mustafa Mustafayev
5
ORCID: ORCID
Zhumagali Ospanbayev
1
Aiman Omarova
1
Adil Kusmangazinov
4

  1. Kazakh Research Institute of Agriculture and Plant Growing, Laboratory of Agriculture, Erlepesov St, 1, 040909, Almalybak, Kazakhstan
  2. International Platform for Dryland Research and Education, Tottori University, Tottori, Japan
  3. International Center for Biosaline Agriculture (ICBA), Dubai, UAE
  4. Al-Farabi Kazakh National University, Department of Biodiversity and Bioresources, Almaty, Kazakhstan
  5. Azerbaijan National Academy of Science, Institute of Soil Science and Agrochemistry, Baku, Azerbaijan
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Abstract

Clarifying the genetic background of the drought-tolerance trait is a crucial task that may help to improve plant performance under stress by a genetic engineering approach. Dehydration-responsive element-binding protein (DREB) is a transcription factor family which modulates many stress-responsive genes. In this study, we isolated a DREB homolog gene named ZmDREBtv from Zea mays var. Tevang-1. Using bioinformatic tools, a number of InDels and SNPs in ZmDREBtv sequence different from the reference accession were identified. In addition, based on deduced protein sequence similarity, ZmDREBtv was assigned to transcription factor DREB2 class as featured by a conserved DNA binding domain - AP2. The ZmDREBtv construct under thecontrol of the rd29A promoter was transformed into a drought-sensitive maize plant, K7 line. The transgenic plants were assessed with reference to molecular and phenotypic characteristics related to the drought-tolenrance trait. The results proved that the maize plants carrying ZmDREBtv gene showed enhanced tolerance and better performance to the water-deficit environment at different stages, compared to the wild-type plants.

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Authors and Affiliations

Thi Thu Hue Huynh
Thuy Linh Nguyen
Han Ly Luu
Hai Ha Nguyen
Hong Diep Le
Manh Minh Bui
Thi Hang Pham
Thi Bich Thao Doan
Thi Thu Hien Le
Hong Hanh Ha
Van Hai Nong

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