Abstract
The paper presents results of research concerning possibilities of
applications of reporter-genes based microorganisms, including the
selective presentation of defects and advantages of different new
scientific achievements of methodical solutions in genetic system
constructions of biosensing elements for environmental research. The most
robust and popular genetic fusion and new trends in reporter genes
technology – such as LacZ (β-galactosidase), xylE (catechol
2,3-dioxygenase), gfp (green fluorescent proteins) and its mutated forms,
lux (prokaryotic luciferase), luc (eukaryotic luciferase), phoA (alkaline
phosphatase), gusA and gurA (β-glucuronidase), antibiotics and heavy
metals resistance are described. Reporter-genes based biosensors with use
of genetically modified bacteria and yeast successfully work for
genotoxicity, bioavailability and oxidative stress assessment for
detection and monitoring of toxic compounds in drinking water and
different environmental samples, surface water, soil, sediments.
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Authors and Affiliations
Stanisław Józef Rosochacki