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Abstract

The present study investigated the potential use of the nano-emulsion of Lippia multiflora Mold. essential oil in managing the cabbage pest ( Brassica oleracea L.) in two Ivorian areas (Yamoussoukro and Korhogo) during the wet seasons (April-September 2018). The nano- -emulsion was tested against cabbage diamondback moth ( Plutella xylostella), aphid ( Brevicoryne brassicae), webworm ( Hellula undalis), cutworm ( Spodoptera exigua) and whitefly ( Bemisia tabaci) under field conditions. The efficacy of essential oil emulsion was compared with the synthetic pesticide Karate 5 EC (Lambda cyhalothrin 52 g · l–1). The results indicated that the nano-emulsion of essential oil gave better control of the cabbage insect pest than the untreated plots. For all the insects studied, the nano-emulsion was very effective towards the species B. brassicae and P. xylostella for which the reduction of the mean population was respectively, 28.48 ± 0.2 and 0.6 ± 0.02 in Yamoussoukro and 0.0 and 7.11 ± 0.16 in Korhogo, compared to 45.32 ± 0.43 and 15.89 ± 0.23, respectively, for untreated plots. The yields of cabbage heads obtained in both areas Yamoussoukro and Korhogo were 4.7 and 15, respectively. The head damage percentages were 23.3% in Yamoussoukro and 26.7% in Korhogo when the fields were sprayed with nano-emulsion and were 13.3% and 28.3%, respectively, when the cabbages were treated with the synthetic pesticide. The formulation obtained here might be an interesting alternative for integrated pest management of cabbage.
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Authors and Affiliations

Vama Etienne Tia
1
Soumahoro Gueu
2
Mohamed Cissé
1
Yalamoussa Tuo
3
Ayekpa Jean Gnago
4
Eugène Konan
5

  1. Département Biochimie – Génétique, Université Peleforo Gon Coulibaly, BP1328 Korhogo, Côte d’Ivoire (Ivory Coast)
  2. Laboratoire des Procédés Industriels de Synthèse, de l’Environnement et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix Houphouët Boigny, BP1093 Yamoussoukro, Côte d’Ivoire (Ivory Coast)
  3. Département Biologie Animale, Université Peleforo Gon Coulibaly, BP1328 Korhogo, Côte d’Ivoire (Ivory Coast)
  4. Laboratoire de Zoologie Agricole et d’Entomologie, Institut National Polytechnique Félix Houphouët-Boigny, BP1093 Yamoussoukro, Côte d’Ivoire (Ivory Coast)
  5. Département de Recherche et Développement, Compagnie Ivoirienne de Coton (COIC), BP193 Korhogo, Côte d’Ivoire (Ivory Coast)
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Abstract

Our research provides novel information concerning the insecticidal activity of Brassica alba mustard oil applied to the intestinal tract via insects’ diet against pests from the order Lepidoptera: Cydia pomonella, Dendrolimus pini, and Spodoptera exigua. The LC50 value of the oil against C. pomonella was 0.422 mg ⋅ ml–1. The LC50 of the plant oil against D. pini was 11.74 mg ⋅ ml–1. The LC50 of the botanical product against S. exigua was 11.66 mg ⋅ ml–1. The plant substance was the most active against C. pomonella in comparison with D. pini and S. exigua. The LC50 values of the oil against D. pini and S. exigua were similar. The plant oil exhibited high insecticidal activity against pests from the order Lepidoptera and may prove to be an effective biopesticide.
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Authors and Affiliations

Edyta Konecka
Adam Kaznowski
Wirginia Marcinkiewicz
Damian Tomkowiak
Mirosław Maciąg
Małgorzata Stachowiak
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Abstract

As alternatives to chemical insecticides, entomopathogenic fungi or wild plants and their secondary metabolites are being used. These biocontrol agents are significant because of their biodegradability, specificity, eco-friendliness, and utility as agents to reduce insecticide resistance. In this study five ethyl acetate extracts of locally isolated fungal strains ( Talaromyces atroroseus, Fusarium chlamydosporum, Talaromyces stipitatus, Trichoderma lixii, Beauveria bassiana) as well as alkaloid extract of Haloxylon salicornicum were extracted and investigated as biocontrol agents against cotton mealybug Phenacoccus solenopsis. The results indicated that all extracts had toxic effects against P. solenopsis except the extract of T. stipitatus. The LC50 values and toxicity index indicated that the alkaloid extract of H. salicornicum was the most toxic one (26 ppm) after 72 hours of treatment followed by the extracts of F. chlamydosporum (77 ppm), then B. bassiana (84 ppm) and T. lixii (118 ppm). On the other hand, there were significant changes in tested insect enzyme activities (amylase, lipase, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and acetyl choline esterase (AchE) as well as total proteins and lipids in the insects treated with the alkaloid extract of H. salicornicum, and ethyl acetate extracts of F. chlamydosporum and B. bassiana after 24 hours of treatment compared to the control. GC/MS analyses of fungal extracts indicated that there were some bioactive compounds like hexadecanoic acid, octadecanoic acid, and tetradecanoic acid. In addition, the anabasine compound was found as a major constituent of the alkaloid extract of H. salicornicum and identified by 1H NMR and GC/MS analysis. In conclusion, according to this study, it was recommended that the alkaloid extract of H. salicornicum and the ethyl acetate extracts of F. chlamydosporum, B. bassiana, and T. lixii be used as alternatives to chemical insecticides for controlling the cotton mealybug P. solenopsis.
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Authors and Affiliations

Reda R.H. Abdullah
1
Ahmed Ramadan El-Rokh
2

  1. Cotton Pesticides Evaluation Research Department, Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt
  2. Piercing Sucking Pests Research Department, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
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Abstract

The efficacy of the fungus Lecanicillium lecanii and two bacteria, Bacillus thuringiensis and Streptomyces avermitilis against the two-spotted spider mite Tetranychus urticae Koch and side effects on its predatory mite Phytoseiulus persimilis A.-H. was studied under laboratory conditions. Both S. avermitilis and B. thuringiensis based biopesticides resulted in maximum mortality rates of 90–100% and 91–99% for spider mite adults and larvae, respectively. The mortality of spider mite larvae under fungus L. lecanii treatment was around 60%. These bacteria and fungus also had toxic effects against P. persimilis on the same day of applying insecticides and releasing the predatory mite. The release of predatory mites one day post-treatment of plants with L. lecanii and 7 days post-treatment with B. thuringiensis or S. avermitilis did not negatively affect the survival of predators released. These findings support the potential use of entomopathogenic fungi and bacteria in combination with predatory mites in spider mite biocontrol.

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Authors and Affiliations

Alexandra A. Zenkova
Ekaterina V. Grizanova
Irina V. Andreeva
Daria Y. Gerne
Elena I. Shatalova
Vera P. Cvetcova
Ivan M. Dubovskiy

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