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Abstract

The last biomonitoring study in Poland on intoxication with fluoride compounds of deer was conducted almost two decades ago. Given the fact that fluoride level in air and water is not widely monitored in Poland, it is justified to undertake monitoring of F- levels in people and other long-lived mammals. This paper provides the assessment of the present level of fluoride accumulation in mineralized tissue of large herbivorous mammals. The aim of the present study was to determine fluoride concentration in teeth of deer inhabiting the areas of Poland which are industrially uncontaminated with fluoride compounds, to establish possible correlations between the analysed parameters, and to provide a comparison of the present results with those obtained in other studies. Mean concentration of fluoride in all analysed samples amounted to 231.0 F mg/kg, with the minimum value of 22.0 F mg/kg and the maximum of 935.0 F mg/kg. This results from the development of industry and a widespread use of fluoride-supplemented caries prevention products which contributes to an intense accumulation of these substances in vertebrates, predominantly in mineralized tissue.
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Authors and Affiliations

M. Palczewska-Komsa
1
K. Barczak
1
K. Grocholewicz
2
J. Buczkowska-Radlińska
1
P.R. Piotrowski
3
E. Sobolewska
4

  1. Pomeranian Medical University in Szczecin, Department of Conservative Dentistry and Endodontics, Powstańców Wlkp. 72, 71-111 Szczecin, Poland
  2. Pomeranian Medical University in Szczecin, Integrated Dentistry Department, Powstańców Wlkp. 72, 71-111 Szczecin, Poland
  3. University of Medical Sciences in Poznań, Professor Emeritus of the Medical Faculty, Bukowska 70, 60-812 Poznań, Poland
  4. Pomeranian Medical University in Szczecin, Department of Dental Prosthetics, Powstańców Wlkp. 72, 71-111 Szczecin, Poland
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Abstract

Glyphosate is an inhibitor of the shikimate pathway in plants and the most widely used broad-spectrum herbicide. Due to the abundance of its use, there exists a necessity to measure the levels both in humans and in the environment to control the nefarious outcomes of its use. The appropriateness, selectivity, and the specifi city of the employed analytical methods are crucial for the reliability of the resultant deductions when conducting biomonitoring studies on possible exposure to chemicals, whether the samples are biological or environmental in nature. The aim of this study is to evaluate the analytical techniques used to monitor glyphosate levels in human and environmental samples. A detailed web-based literature search was conducted to gather data on the analytical techniques used for glyphosate determination. The most preferred authentic samples are blood, urine, and milk. Environmental samples include plants, soil, and water. Among widely used analytical techniques used to detect glyphosate are High Performance Liquid Chromatography, Liquid Chromatography with tandem mass spectrometry, Gas Chromatography – Tandem Mass Spectrometry, and enzyme-linked immunosorbent assay. Depending on the sample and study, the most suitable analytical method has been selected. A critical evaluation and publication of pre-existing literature on analytical methods in glyphosate-based herbicide detection will thus aid all relevant researchers in the determination of an appropriate, selective, and specific methodology
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Authors and Affiliations

Kumsal Kocadal
1
ORCID: ORCID
Fehmi Burak Alkas
2
ORCID: ORCID
Dilek Battal
2 3
ORCID: ORCID
Sahan Saygi
3

  1. Near East University, Faculty of Pharmacy, Department of Toxicology, Cyprus
  2. Mersin University, Faculty of Pharmacy, Department of Toxicology, Cyprus
  3. Near East University, Faculty of Pharmacy, Department of Toxicology, Turkey
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Abstract

Polycyclic aromatic hydrocarbons (PAHs) are organic compounds characterized by carcinogenic, toxic and mutagenic effect on life organisms. The mining and burning of coal are widely practiced in the tundra zone which results in the release of PAHs. The studies of PAHs content in organogenic horizon of tundra soil and southern tundra plants were performed at the control sites and at areas affected by coal industry. The soil and plants were analyzed for PAHs by HPLC. It was established that tundra soils, lichens and mosses are contaminated with polyarenes to a larger extent in the areas affected by the coal mining. The peak of PAHs concentration in the area affected by the Vorkutinskaya coal mine was observed within the distance of 0.5 km, and within 1.0 km in the area affected by the thermal power station. We propose to use diagnostic correlations of fluoranthene/ chrysene and fluoranthene/benz[b]fluoranthene in soils and mosses Pleurozium schreberi (Brid.) Mitt. to characterize the origin of polyarenes in tundra ecosystems. The similar polyarenes distribution is found in the soils and plants under the influence of coal industry. With polyarenes supply from industrial sources decreasing, their bioaccumulation level in the plants is reduced. We recommend Pleurozium schreberi to be used as a pollution indicator of tundra phytocenoses with PAHs and leaves of Betula nana L. for assessment of short-time changes of polyarene contents. The general contents rather than the surface accumulations are more suitable for the monitoring studies.

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Authors and Affiliations

Evgenia Yakovleva
Dmitriy Gabov
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Abstract

Variability of stress proteins concentration in caged carp exposed to transplantation experiment model dam reservoir was caused only by natural (climatic and biological) conditions. Thus, the reference data of stress proteins concentration range in young carp individuals were obtained. Metallothionein, HSP70 and HSP90 protein concentrations as biomarkers were assayed in the livers, gills and muscles of six-month-old (summer) or nine-month-old (autumn) carp individuals in relation to the site of encaging, season (summer or autumn), the term of sampling (1, 2 or 3 weeks after the transplantation) and tissue. Physicochemical analyses of the condition of water as well as pollution detection were conducted during each stage of the experiment. As the result of this study, the range of the variability of the stress protein concentration in young carp individuals was obtained. According to the analyses of the aquatic conditions of a reservoir with no detectable pollutants, we conclude that the variability in the stress protein concentration levels in the groups that were compared is solely the result of the natural conditions. Future regular monitoring of the reservoir using the transplantation method and young carp individuals will be both possible and reliable. Moreover, the range of variability in the stress protein concentrations that were measured in the young C. carpio individuals acquired from the model dam reservoir in relation to all of the studied factors may be applied in the monitoring of any other similar reservoir.
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Authors and Affiliations

Agnieszka Babczyńska
1
Monika Tarnawska
1
Piotr Łaszczyca
1
ORCID: ORCID
Paweł Migula
1
Bartosz Łozowski
1
Andrzej Woźnica
1
Ilgiz Irnazarow
2
ORCID: ORCID
Maria Augustyniak
1

  1. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Poland
  2. Institute of Ichtyobiology and Aquaculture in Gołysz, Polish Academy of Sciences, Poland
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Abstract

The results of a research into the scale and consequences of the degradation of aquatic ecosystems in Ukrainian Polesie have been detected in article, and the areas of increased anthropogenic pressure have been identified which greatly affect the condition and number of aquatic macrophytes. The biodiversity of sites with different anthropogenic load was evaluated using the biodiversity criteria. In the research, the structural and functional features of macrophytic species diversity within Teteriv River ecological corridor as a typical river landscape of Ukrainian Polesie were determined and described, the floristic composition was determined. Within the ecological zones, the number of species and their projective coverage in areas with different anthropogenic pressures within Teteriv River ecological corridor were determined. The basic criteria for the implementation of deferred biomonitoring based on the analysis of the dynamics of the spe-cies composition of the phytocoenoses of Teteriv River ecological corridor on the indicators of ecological stability and plasticity using the species-specific criteria, are: Margalef species richness index, Sørensen–Dice index, Shannon diversity index, Simpson’s index, and Pielou’s evenness index. Based on the results, correlation dependencies have been constructed, which will allow to obtain data on the stability of the development of aquatic ecosystems according to the data of species surveys. Interconnections between biodiversity indicators and indicators of surface water quality within the Ukrainian Polesie were found; they are the fundamental component of a long-term monitoring of the stability in the development of aquatic phytocenoses.

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Authors and Affiliations

Tetiana P. Fedonyuk
Roman H. Fedoniuk
Anastasiia A. Zymaroieva
Viktor M. Pazych
Ella O. Aristarkhova
ORCID: ORCID

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