Monoculture of modern cereal crops are popular due to the technical and organizationa lreasons. They are easier in crop husbandry, qua lity and product use. However, in monoculture chemical protection of crops is a norm, due to the fact that they are more susceptible to diseases, pests and sometimes weed infestation. In order to keep high and stable grain yields and quality in monoculture one has to use high inputs. Experimentally and practically it has been proved that cultivar and species mixtures can constitute an alternative to cultivar growing in pure stands. It has been found that in mixtures opperate different epidemiological and ecological factors, which lead to considerable disease reduction, pest and weed control, which finally result in higher and more stable grain yields than in barley varietes grown in pure stands. The results of two years field experiment designed to evaluate epidemiological and economical effects of winter barley cultivar mixtures are presented. The studies were carried out in two sites – experimental lStation for Variety Testing Słupia Wielka (in Wielkopolska region) and Plant Breeding Station Bąków (Opole District). This two sites were 300 km away from each other, and had different soi land meteorological conditions. In the experiment impact of different barley cultivars and their different two- and three-component mixtures were tested with reduced dosages of fungicidies on grain yield in the mixtures compared with pure stands were evaluated.

Powdery mildew caused by Blumeria graminis f. sp. hordei is one of the most important diseases of barley in Poland. B. graminis is a genetically diverse pathogen with different special forms and races. The aim of the two-years’ experiment was to assess of B. gramins f. sp. hordei virulence frequency and powdery mildew occurrence on four winter barley cultivars. Virulence frequency of the pathogen depended on place and term of exposition. The occurrence of powdery mildew on four winter barley cultivars depended on virulence frequency of the pathogen and weather conditions.

It is challenging to obtain proper leaf wetting. An angled spray could overcome this impediment, but which spray angle is best suited to droplet size is still unknown. In an outdoor pot experiment, seven doses of cycloxydim and sethoxydim were sprayed with single-orifice standard, anti-drift, and air induction (having a fine, medium, and extremely coarse spray quality, respectively) flat fan nozzles, using spray angles of 10°, 20° backward, 0° (vertical), 10°, 20°, 30°, 40°, 50°, and 60° forward relative to the direction of nozzle trajectory on wild barley at the three-leaf stage. Generally, the forward angled spray was better than the backward angled spray. With a standard flat fan nozzle, the forward angling of spray from 0° to 20° reduced the ED50 from 60.24 to 39.85 g a.i. ⋅ ha−1 for cycloxydim and from 150.51 to 81.13 g a.i. ⋅ ha−1 for sethoxydim. With an anti-drift flat fan nozzle, the forward angling of spray from 0° to 30° reduced the ED50 from 72.57 to 50.20 g a.i. ⋅ ha−1 for cycloxydim and from 181.94 to 104.51 g a.i. ⋅ ha−1 for sethoxydim. With an air induction flat fan nozzle, the forward angling of spray from 0° to 40° reduced the ED50 from 102.96 to 45.52 g a.i. ⋅ ha−1 for cycloxydim and from 209.91 to 92.80 g a.i. ⋅ ha−1 for sethoxydim. More angling did not improve the efficacy of these herbicides. Our results revealed that larger spray droplets needed more spray angle than smaller spray droplets to achieve an equal control.

In the four-year experiment the impact of four different barley varieties and selected two-and three-component mixtures were tested. The studies were carried out at two sites: Experimental Station for Variety Testing Słupia Wlk. (Wielkopolska region) and Plant Breeding Station Bąków (Opole District) during four growing seasons (2001/2002–2004/2005). The aim of the studies was to evaluate the yield through growing barley variety mixtures in combination with reduced use of fungicide and application dose rates. Positive effects (1–15% yield increase in mixtures compared to pure stands) were observed. On the base of obtained results it can be stated that winter barley variety mixtures can constitute an alternative way of growing winter barley, especially at low-input and ecological agriculture.

Barley scald, caused by Rhynchosporium commune is one of the most prevalent diseases in barley (Hordeum vulgare L.) worldwide. The primary loss from scald is reduced yield, which can exceed 25% in dry areas. In our earlier studies, we developed a low-resolution linkage map for recombinant inbred lines of the cross Tadmor/WI2291. Quantitative trait loci (QTLs) for scald were localized on chromosomes 2H and 3H flanked by Simple Sequence Repeat (SSR) markers HVM54 and Bmac0093b on 2H and HVLTPP8, HVM62 and Bmag0006 on 3H. These chromosome 3H markers were found to be located close to the Rrs1 − R. commune resistance gene(s) on chromosome 3H. In this study, 10 homozygous resistant and 10 homozygous susceptible plants each from the F7 population of Tadmor/ Sel160, a panel of 23 barley varieties used routinely in the International Centre for Agricultural Research in the Dry Areas (ICARDA) breeding program and three populations were used for scald resistance screening using 25 DNA markers that are located very close to scald resistance gene(s) on barley chromosomes. Only five of those markers clearly discriminated co-dominantly between resistant and susceptible plants. These markers, Ebmac0871- SSR, HVS3-SCAR, Bmag0006-SSR, reside on different arms of barley chromosome 3H. Ebmac871 is localized on the short arm of 3H and HVS3 and Bmag0006 are localized on the long arm of 3H. This result indicates that the scald resistance genes which they tag are probably close to the centromeric region of this chromosome. Scald resistance from several sources map to the proximal region of the long arm of chromosome 3H, forming the complex Rrs1 locus. The availability of highly polymorphic markers for the discrimination of breeding material would be extremely useful for barley breeders to select for the trait at the DNA level rather than relying on phenotypic expression and infection reaction.

In the four-year experiment the impact of four different winter barley varieties and selected two- and three-component mixtures were tested. Reduced dosages of fungicides on disease reduction in the mixtures compared with pure stands were evaluated. The studies were carried out at two sites: Experimental Station for Variety Testing Słupia Wlk. (Wielkopolska region) and Plant Breeding Station Bąków (Opole District). Observations on powdery mildew occurrence during the vegetation season were done every 7–10 days. In order to compare the disease occurrence levels on different cultivars in pure stands and on their mixtures combined with different fungicide treatments the Area Under Disease Progress Curve (AUDPC) was evaluated. On the base of the AUDPC values the reduction of powdery mildew occurring in the mixtures due to epidemiological and ecological factors functioning in mixed stands were also evaluated. On the base of obtained results it can be stated that winter barley variety mixtures combined with different fungicide treatments do reduce the powdery mildew incidence comparing to pure stands and winter barley variety mixtures can constitute an alternative way of growing winter barley, especially at low-input and ecological agriculture.

Stem base health of spring barley cultivated under organic, integrated and conventional systems and fungal communities were studied. A worst plant health status was observed in the organic system. The macroscopic and subsequent mycological analyses revealed the occurrence of Bipolaris sorokiniana and Fusarium spp. The incidence of B. sorokiniana on stem bases was clearly dependent on a farming system, and the highest incidence of this pathogen was observed in the organic system. Also, in that system, Fusarium spp. were isolated more numerously in the beginning of tillering, but in dough stage B. sorokiniana was the most prevalent pathogen, and Fusarium spp. were more numerous in integrated and conventional systems. It is worth to note that organic conditions could be favourable to Gliocladium spp. Because of growing interest in ecology, excluding the use of pesticides and increasing popularity of biological disease control, these antagonistic fungi could be useful in organic systems.

Barley phylloplane is seriously colonized by Drechslera graminea, the causal agent of leaf

stripe disease in the hos. The present study involved the elucidation of alterations induced

in the protein content of the host due to Drechslera infection. Naturally growing barley

plants were obtained from fields and Drechslera graminea was isolated and identified from

diseased plants’ leaves. After identification and preparation of the pure culture, the pathogen

was inoculated on plants grown under aseptic and controlled laboratory conditions.

Changes in the total soluble cytoplasmic proteins and defense enzymes of the host such

as polyphenol oxidase (PPO), peroxidase (POX), phenylalanine lyase (PAL) and tyrosine

ammonia lyase (TAL) were observed up to 5 h after inoculation. The results demonstrated

a significant effect of the pathogen on the cytoplasmic protein expression of the host as well

as in its defense system.

In the spring of 2019, many plants, mainly winter wheat, were observed to have dwarfism and leaf yellowing symptoms. These plants from several regions of Poland were collected and sent to the Plant Disease Clinic of the Institute of Plant Protection – National Research Institute in Poznań to test for the presence of viral diseases. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) results showed numerous cases of Wheat dwarf virus (WDV) and a few cases of plant infections caused by Barley yellow dwarf viruses (BYDVs). WDV was detected in 163 out of 236 tested winter wheat plants (69.1%), in 10 out of 27 tested winter barley plants (37%) and in 6 out of 7 triticale plants (85.7%) while BYDVs were found, respectively, in 9.7% (23 out of 236) and in 18.5% (5 out of 27) of tested winter forms of wheat and barley plants. Infected plants came mainly from the regions of Lower Silesia and Greater Poland. Furthermore, individual cases of infections were also confirmed in the following districts: Lubusz, Opole, Silesia, Kuyavia-Pomerania and Warmia-Masuria. Results of Duplex-immunocapture-polymerase chain reaction (Duplex-IC-PCR) indicated the dominance of WDV-W form in wheat and WDV-B form in barley plants. Moreover, results of reverse transcription – polymerase chain reaction (RT-PCR) connected with restriction fragment length polymorphism (RFLP) analysis, performed for 17 BYDVs samples, revealed 8 BYDV-PAS, 4 BYDV-MAV and 2 BYDVPAV as well as the presence of two mixed infections of BYDV-MAV/-PAS and one case of BYDV-MAV/-PAV. Next, RT-PCR reactions confirmed single BYDV-GAV infection and the common presence of BYDV-SGV. To the best of our knowledge, in 2020 the viruses were not a big threat to cereal crops in Poland.