This paper presents an investigation into effect of nitrogen content of shielding gas mixture on weld geometry, microstructure and hardness of pulsed laser welded 2205 duplex stainless steel. Full penetration autogenous welding was performed on 2 mm thick plates using a low power pulsed Nd: YAG laser. light and scanning electron microscopy were used to study the resulting microstructures. It is observed that 2 mm full penetrated joint decreases to 1.8 mm by dominating nitrogen in argon-nitrogen mixture. Different morphologies of austenite phase as well as an increase of 8% of its content can be observed in pure nitrogen shielded welds. Average weld grain size in sample which is welded in nitrogen atmosphere stands at approximately 41 μm which is smaller than that of argon atmosphere which is about 51 μm. Micro-hardness test reveals that hardness values increase from 280 HV in base metal to 307 HV in weld center line and the shielding gas mixture does not significantly influence the weld hardness.