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Number of results: 6
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Iterative sliding mode observer for sensorless control of five-phase permanent magnet synchronous motor

D. Zhao J. Yang M. Dou
Bulletin of the Polish Academy of Sciences Technical Sciences | 2017 | 65 | No 6 (Special Section on Civil Engineering – Ongoing Technical Research. Part II) | 845-857 | DOI: 10.1515/bpasts-2017-0092
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Authors and Affiliations

D. Zhao
J. Yang
M. Dou

Molecular cloning, expression, purification and osteoblasts proliferation activity of sika deer thymosin beta10

M. Liu J. Wang D. Zhao Y. Zhao H. Zhang Z. Xu X. Bai
Polish Journal of Veterinary Sciences | 2017 | No 4 | DOI: 10.1515/pjvs-2017-0095
Keywords affinity chromatography cell proliferation prokaryotic expression sika deer thymosin 10
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Authors and Affiliations

M. Liu
J. Wang
D. Zhao
Y. Zhao
H. Zhang
Z. Xu
X. Bai

Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody

G. Dai X. Huang Q. Liu Y. Li L. Zhang K. Han J. Yang Y. Liu F. Xue D. Zhao
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 4 | 579-587 | DOI: 10.24425/pjvs.2022.143541
Keywords goose astrovirus capsid protein monoclonal antibody epitope
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Abstract

Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
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Authors and Affiliations

G. Dai
1 2 3
X. Huang
1 3
Q. Liu
1 3
Y. Li
1 3
L. Zhang
1 3
K. Han
1 3
J. Yang
1 3
Y. Liu
1 3
F. Xue
2
D. Zhao
1 2 4 3
  1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
  2. College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Tongwei Road, Nanjing City, Jiangsu Province 210095, PR China
  3. Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China
  4. Institute of Life Sciences, Jiangsu University, No. 301 Xuefu Road, Zhenjiang, Jiangsu Province, 212013, PR China

Antibody level comparison after porcine epidemic diarrhea vaccination via different immunization routes

C. Hu X. Xie D. Zhao H. Liu X. Liu T. Yang W. Sun
Polish Journal of Veterinary Sciences | 2024 | vol. 27 | No 1 | 143-146 | DOI: 10.24425/pjvs.2024.149342
Keywords porcine epidemic diarrhea IgG IgA neutralizing antibody
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Abstract

Porcine epidemic diarrhea (PED) is a disease extremely harmful to pig health. Intramuscular and Houhai acupoint injections are the main immunization routes to prevent and control PED. This study aimed to evaluate the efficacy of these two routes in pregnant sows based on serum IgG, IgA, and neutralizing antibody levels. PED virus (PEDV) immunoprophylaxis with live-attenuated and inactivated vaccines was administered. The vaccinations for the intramuscular injections elevated IgG and neutralizing antibody levels more than Houhai acupoint injections at most timepoints after immunization. However, the anti-PEDV IgA antibodies induced by vaccination with the two immunization routes did not differ significantly. In conclusion, intramuscular injections are better than Houhai acupoint injections for PEDV vaccination of pregnant sows.
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Bibliography

1. Brown J, Poonsuk K, Cheng TY, Rademacher C, Kalkwarf E, Tian L, McKeen LA, Wang C, Gimenez-Lirola L, Baum D, Karriker LA (2023) Comparison of two diagnostic assays for the detection of serum neutralizing antibody to porcine epidemic diarrhea virus. Animals (Basel) 13:757.
2. Hsueh FC, Chang YC, Kao CF, Hsu CW, Chang HW (2020) Intramuscular immunization with chemokine-adjuvanted inactive porcine epidemic diarrhea virus induces substantial protection in pigs. Vaccines (Basel) 8:102.
3. Jin H, Wu Y, Bi S, Xu Y, Shi F, Li X, Ma X, Hu S (2020) Higher immune response induced by vaccination in Houhai acupoint relates to the lymphatic drainage of the injection site. Res Vet Sci 130: 230-236.
4. Jung K, Saif LJ, Wang Q (2020) Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and pre-vention and control. Virus Res 286: 198045.
5. Krishna VD, Kim Y, Yang M, Vannucci F, Molitor T, Torremorell M, Cheeran MC (2020) Immune responses to porcine epidemic diar-rhea virus (PEDV) in swine and protection against subsequent infection. PLoS One 15: e0231723.
6. Langel SN, Paim FC, Alhamo MA, Buckley A, Van Geelen A, Lager KM, Vlasova AN, Saif LJ (2019) Stage of gestation at porcine epidemic diarrhea virus infection of pregnant swine impacts maternal immunity and lactogenic immune protection of neonatal suckling piglets. Front Immunol 10: 727.
7. Lee C (2015) Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus. Virol J 12: 193.
8. Lv C, Xiao Y, Li X, Tian K (2016) Porcine epidemic diarrhea virus: current insights. Virus Adapt Treat 8: 1-12.
9. Shibata I, Tsuda T, Mori M, Ono M, Sueyoshi M, Uruno K (2000) Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages. Vet Microbiol 72: 173-182.
10. Sun D, Wang X, Wei S, Chen J, Feng L (2016) Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review. J Vet Med Sci 78: 355-363.
11. Xu W, Hu S (2021) Administration of infectious bursal disease vaccine in Houhai acupoint promotes robust immune responses in chickens. Res Vet Sci 142: 149-153.
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Authors and Affiliations

C. Hu
1
X. Xie
2
D. Zhao
3
H. Liu
1
ORCID: ORCID
X. Liu
4
T. Yang
5
W. Sun
6
  1. Pulike Biological Engineering Inc., Luoyang, Henan, 471000, China
  2. Yiyang Vocational and Technical College, Yiyang, Hunan, 413055, China
  3. College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, 410128, China
  4. Xiangtan Center for Animal Disease Prevention and Control, Xiangtan, Hunan, 411104, China
  5. College of Life Sciences and Resource Environment, Yichun University, Yichun, Jiangxi, 336000, China
  6. Sinopharm Animal Health Corporation Ltd., Wuhan, Hubei, 430075, China

Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein

T. Xu Y. Liu K. Han D. Zhao J. Yang Y. Li Q. Liu X. Huang K. Bi
Polish Journal of Veterinary Sciences | 2016 | No 4 | DOI: 10.1515/pjvs-2016-0109
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Authors and Affiliations

T. Xu
Y. Liu
K. Han
D. Zhao
J. Yang
Y. Li
Q. Liu
X. Huang
K. Bi

Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings

Y. Li Q. Liu T. Xu X. Huang X. Liu K. Han Y. Liu J. Yang D. Zhao K. Bi W. Sun
Polish Journal of Veterinary Sciences | 2018 | vol. 21 | No 2 | 389–396 | DOI: 10.24425/122613
Keywords goose-derived duck-derived chicken-derived Tembusu virus pathogenicity
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Abstract

In order to compare the pathogenicity of different Tembusu virus (TMUV) strains from geese, ducks and chickens, 56 5-day-old Cherry Valley ducklings which were divided into 7 groups and infected intramuscularly with 7ยด105 PFU/ml per duck of six challenge virus stocks. The clinical signs, weight gain, mortality, macroscopic and microscopic lesions, virus loads in sera of 1, 3, 5, 7, 11 and 14 dpi and serum antibody titers were examined. The results showed that these viruses could make the young ducks sick, but the clinical signs differed with the different species-original strains. All the experimental groups lose markedly in weight gain compared to the control, but there were no obvious distinctions in weight gains, as well as macroscopic and microscopic lesions of dead ducks between the infected groups. However, the groups of waterfowl-derived strains (from geese and ducks) showed more serious clinical signs and higher relative expressions of virus loads in sera than those from chicken-derived. The mortality of waterfowl groups was 37.5%, and the greatest mortality of chicken groups was 12.5%. The serum antibodies of the geese-species group JS804 appeared earlier and were higher in the titers than others. Taken toghter, the pathogenicity of waterfowl-derived TMUV was more serious than chicken-derived TMUV and JS804 could be chosen as one TMUV vaccine strain to protect from the infection.
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Authors and Affiliations

Y. Li
Q. Liu
T. Xu
X. Huang
X. Liu
K. Han
Y. Liu
J. Yang
D. Zhao
K. Bi
W. Sun

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