In this research work, Ti6Al4V alloy material was subjected to electric discharge machining (EDM) and its fatigue life was investigated at low cycle fatigue mode. In order to evaluate the influence of recast layer generated during the machining process on the fatigue life, samples prepared using end milling process were also subjected to similar tests and a comparative analysis is presented. Data were observed in the fully reversed fatigue mode at room temperature using samples fabricated as per ASTM standard E606. The specimen were machined on a spark electric discharge die sink machine which were subjected to fatigue, and the recorded fatigue lives were compared with the fatigue life of end milled specimen. The machined surfaces were examined through optical and scanning electron microscopes, and the roughness was measured with a standard profilometer. It was observed that when the discharge current is augmented, the recast layer formed was in the range of 20 to 70 µm thick. From the results, it is being concluded that fatigue life of the samples fabricated by EDM is less for various load conditions when compared with that of the end milled sample. The milled sample at 160 MPa load exhibited 2.71×105 cycles, which is 64% more when compared to EDM sample.