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Abstract

Przedmiotem dwuletnich badań było poznanie dynamiki rozwoju wybranych grup drobnoustrojów (mikroorganizmy oligotroficzne, kopiotroficznc, proteolityczne, celulolityczne, rozpuszczające fosforany) w glebie nawożonej komunalnymi osadami ściekowymi. Celem przeprowadzonego doświadczenia było wyjaśnienie możliwości zachwiania równowagi biologicznej gleby, przejawiającej się wzmożonym i długotrwałym rozwojem analizowanych grup drobnoustrojów w glebie, po wprowadzeniu do niej materii organicznej w formic osadów ściekowych, w różnych dawkach. W doświadczeniu zastosowano cztery obiekty badawcze: kontrola-gleba + NPK, 2 Mg s.m. osaduha+rok' + NPK, 4 Mg s.m. osadu-hal-rok' + NPK oraz 8 Mg s.m. osaduhatrok' + NPK). Fosfor i potas stosowano przedsiewnie pod orkę, natomiast azot: część przedsiewnie i drugą część pogłównie. Osady ściekowe stosowano przedsiewnie. Poletka glebowe, na których przeprowadzono badania obsiano żytem odmiany Wibro (2003 r.) oraz obsadzono ziemniakami odmiany Bila (2004 r.). Wykazano, że zastosowane dawki osadów ściekowych nic wpływały istotnie statystycznie na namnażanie się mikroorganizmów glebowych. Na podstawie przeprowadzonych analiz mikrobiologicznych stwierdzono, że w badanym okresie (2003-2004) liczebność oznaczonych grup drobnoustrojów ulegała wahaniom, zależnym od terminu pobierania próbek glebowych. Próbki glebowe, niezbędne do przeprowadzenie analiz, pobierane były w lenninach związanych z kolejnymi fazami rozwojowymi roślin. Kolejnym czynnikiem wpływającym na dynamikę rozwoju mikroorganizmów glebowych był gatunek rośliny użytej w doświadczeniu. Żyto uprawiane w 2003 r. stymulowało rozwój większości grup drobnoustrojów (mikroorganizmów oligotroficznych, kopiotroficznych, proteolitycznych, rozpuszczających fosforany). Z kolei ziemniaki w okresie rozwoju generatywnego ( 16.07- 28.08.2004) spowodowały silniejszy rozwój mikroorganizmów celulolitycznych.
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Authors and Affiliations

Agnieszka Wolna-Maruwka
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Abstract

The paper contains a micobiological characteristic of sewage sludge composted in controlled conditions together with bio-wastes (straw, sawdust, bark). An experiment was carried out in which the composted material was mixed up in adequate weight proportion and placed in biorcactor chambers with a constant air flow. The composting process aimed at defining the development dynamics and the survival of pathogenic microorganisms in the sewage sludge composted with different additions in a cybernetic bioreactor. Samples of compost necessary for microbiological analyses were taken at the same time, in reference to the actual temperature value. Bacteriological studies were carried out on selected substrates by plate method determining the number of pathogenic bacteria from the species: Salmonella, Clostridium perfringens, as well as from Enterobacteriaccac family. In the experiments, the presence of living eggs or intestinal ATT pathogens was determined by floatation method, as well. Il was found that the sewage sludge used in composting process did not contain any Salmonella spp. bacteria or any living eggs of intestinal ATT pathogens. Composting process completely eliminated the number or bacteria from Enterobactcriaccae family, bul it did not contribute lo the elimination of Clostridium perfringens bacteria. On the basis of the obtained results, it was found that the elimination of the studied groups of microorganisms, in all studied composts took place with the increase of temperature. In the case or Enterobacteriaceae, it was found that their complete removal from the composted material took place in chamber K3, while in the remaining chambers, it followed 48 hours later. Elimination ofthe vegetative forms ofC!oslridium perfringens bacteria followed after 96 hours of composting, in all composts at the same time. The obtained composts met the sanitary norms according lo the regulations of the EC Commission No. I 85/2007 of February 20, 2007 which changed the regulation of WE No. 809/2003 and WE No. 810/2003 referring to the extension of the validity period of transitional means for composting plants and biogas producing plants according to the instruction orWE No. 1774/2002 of European Parliament and Council and according to the instruction of the Minister for Agriculture and Country Development (2004).
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Authors and Affiliations

Agnieszka Wolna-Maruwka
Jacek Dach
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Abstract

Agricultural utilization of appropriately stabilized sewage sludge appears to be the most rational method of its utilization, even though there is no agreement among scientists as to the impact that these wastes can exert on the maintenance of the soil biological balance. That is why the objective of the performed field-laboratory experiments was to determine the developmental dynamics of selected groups of microorganisms in a grey-brown podzolic soil fertilized with acceptable and unacceptable doses of sewage sludge and farmyard manure. Numbers of six groups of microorganisms were determined at various dates associated with the development of spring barley (total bacterial number, number of actinomycetes, fungi, bacteria from the Azotobacter genus and Pseudomonas j/uorescens) as well as pathogenic bacteria from the Sa/111011ella genus. The selected groups of microorganisms were determined on selective media by the plate method. The obtained research results showed that, in the majority of cases, the applied levels of organic fertilization did not have a significant impact on the numbers of microorganisms in the soil. Therefore, it can be said that the application of both acceptable and unacceptable doses of sewage sludge in the form of fertilizers failed to disturb the biological balance of the examined soil. In addition, the results of the performed experiments indicated that the agricultural utilization of sewage sludge should be forestalled by a sanitation process (e.g. composting) in order to get rid of' pathogenic bacteria, especially bacteria from the Salmonella genus.
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Authors and Affiliations

Agnieszka Wolna-Maruwka
Aleksandra Sawicka
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Abstract

The paper presents microbiological characteristics of sewage sludge derived from the mechanicalbiological sewage treatment plant and farmyard manure as well as composts manufactured from them. In the performed experiment, four types of composts were analyzed. The first of them comprised the sewage sludge alone, the second one - was made up only of farmyard manure, while the remaining composts were prepared by mixing the above-mentioned bio-wastcs in the following proportions: 75% sewage sludge+ 25% farmyard manure and 50% sewage sludge + 50% farmyard manure. The next stage of experiments involved analyses of the composts incubated with soil. The following assays were carried out in the experimental composts and mixtures of soil and composts: counts of Salmonella sp., E. coli, Clostridium perfringens, total counts of bacteria, fungi and actinomycetes on selective media employing the plate method. The performed investigations revealed that the composting process resulted in complete riddance of the Salmonella sp. and reduction in the numbers of the remaining groups of microorganisms. Therefore, it can be said that the composted sewage sludge was suitable for the utilization for agricultural purposes in accordance with the Directive of the Minister of Agriculture and Rural Development of October 2004. Moreover, it was found that, as early as 60 days after the introduction of composts into the soil, counts of the majority of the analyzed groups of microorganisms (with the exception of actinornycctcs and E. coli), including pathogenic bacteria from the C. perfringens genus, were found reduced. The obtained research results proved that the introduction of bi o-wastes into the soil may decrease survivability in the natural environment of certain pathogens; hence it is a good method of utilization of this organic material.
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Authors and Affiliations

Agnieszka Wolna-Maruwka
Jacek Czekała
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Abstract

The paper presents microbiological characteristic of sewage sludge composted in controlled conditions together with biowastes (wheat, maize and rapeseed straw, sawdust and refined glycerol). An experiment was carried out in which the material was mixed at appropriate weight proportions and then placed in bioreactor chambers of constant air flow (4 lźmin-1). The performed composting process aimed at determining the developmental dynamics of heterotrophic bacteria, molds, actinomycetes as well as bacteria from Salmonella genus and Enterobacteriaceae family. Microbiological analyses were performed on selective substrates using Koch's plate method. Moreover, using the floatation method, the presence of live eggs of ATT (Ascaris spp., Trichuris spp., Toxocara spp.) intestinal parasites was assessed and levels of dehydrogenase activity were determined using 1% triphenylotetrazole chloride as a substrate. It was concluded, on the basis of the obtained research results, that the composting process reduced bacterial counts of heterotrophic bacteria, molds and the activity of dehydrogenases activity in all experimental treatments. On the other hand, no reduction was observed in quantities of actinomycetes in the composted materials whose changes in numbers were found to correlate positively most strongly with levels of dehydrogenases activity. In addition, it was found that changes in numbers of the analysed groups of microorganisms depended, primarily, on the pH value and concentrations of ammonia released from the composted materials. Furthermore, the obtained research results also revealed that the sewage sludge used in the experiment did not contain Salmonella spp. bacteria and live eggs of ATT intestinal parasites, and that the composting process reduced completely numbers of bacteria from the Enterobacteriaceae family in all compost treatments. The obtained composts fulfilled all sanitary standards complying with the requirements issued by the Minister of Agriculture and Rural Development (2008) as well as with the EU regulation (EC) No. 185/2007 from February 2007 changing EEC regulations No. 809/2003 and No. 810/2003 connected with the extension of the period of transitional requirements for composting and biogas plants as provided by the EU regulation No. 1774/2002 of the European Parliament and Council.

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Authors and Affiliations

Agnieszka Wolna-Maruwka
Krzysztof Pilarski
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Abstract

The objective of this work was investigation of the growth dynamics of proteolytic bacteria and the enzymatic activity in soil for the cultivation of maize (Zea mays L.), as well as the maize yield under application of some selected organic fertilizers. Intensity and the direction of the developed changes in the soil depended on the type of applied organic fertilizer, the size of its dose introduced into the soil and on the developmental phase of the grown plant (maize). On the basis of obtained results it was found that all tested organic substances stimulated the activity of proteases. Yields ofmaize as raw material for silages obtained from soil fertilized with sewage sludge were similar to those obtained after the application of pig slurry and they exceeded maize yields harvested from other ferti I ization objects.
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Authors and Affiliations

Alicja Niewiadomska
Hanna Sulewska
Agnieszka Wolna-Maruwka
Justyna Klama
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Abstract

Tropospheric ozone is one of the most reactive air pollutants, which causes visible injuries, as well as biomass and yield losses. The negative effect of ozone is cumulative during the growing season; hence crops are the most sensitive plants. Visible symptoms and biomass losses can cause economic losses. Tobacco plants have been recognized as one of the best bioindicators, but data on the cumulative effect of ozone on this species are limited. Results of an experiment with ozone-sensitive tobacco plants grown on sites varying in ozone concentration are presented in this paper. Two indices were used for data presentation of visible leaf injury degree. Higher solar radiation was the main cause of higher ozone concentration at the rural site. Higher tropospheric ozone concentrations were noted in 2010 in comparison to 2011, which was reflected in visible leaf injury. Canonical variate analysis did not reveal highly significant differences between sites, however, differences were observed in certain investigation periods. Moreover, higher leaf injury was noted at the rural site at the end of the experiment in both experimental years. This indicates the cumulative effect of ozone during the growing season. However, higher injury variability was noted at the urban site, even though lower ozone concentrations were noted there. Lower variability of injury at the rural site might suggest lack of influence of particulate matter and occurrence of higher injury even though lower ozone concentrations occurred. Better detection of ozone injury was shown by the first index based on three mean values.
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Authors and Affiliations

Anna Budka
Janina Zbierska
Klaudia Borowiak
Dariusz Kayzer
Agnieszka Wolna-Maruwka
Anita Schroeter-Zakrzewska
Anna Chlebowska
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Abstract

The experiment consisted in monitoring the count of moulds and three selected Trichoderma sp. isolates (T1 - Trichoderma atroviride, T2 - Trichoderma harzianum, T3 - Trichoderma harzianum) in vegetable (onion and tomato) waste composted with additives (straw, pig manure). Additionally, the aim of the study was to determine the type of interaction occurring between autochthonous fungi isolated from composts after the end of the thermophilic phase and Trichoderma sp. strains applied in the experiment. Number of microorganisms was determined by the plate method, next the identification was confirmed. The rating scale developed by Mańka was used to determine the type of interactions occurring between microorganisms. The greatest count of moulds in onion waste composts was noted in the object which had simultaneously been inoculated with two strains T1 - T. atroviride and T3 - T. harzianum. The greatest count of moulds was noted in the tomato waste composts inoculated with T2 - T. harzianum strain. Microscope identification revealed that Penicillum sp., Rhizopus sp., Alternaria sp. and Mucor sp. strains were predominant in onion waste composts. In tomato waste composts Penicillium was the predominant genus, followed by Rhizopus. The test of antagonism revealed the inhibitory effect of Trichoderma isolates on most autochthonous strains of moulds. Tomato waste composts proved to be better substrates for the growth and development of Trichoderma sp. isolates. The results of the study show that vegetable waste can be used in agriculture as carriers of antagonistic microorganisms.

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Authors and Affiliations

Agnieszka Wolna-Maruwka
Tomasz Piechota
Alicja Niewiadomska
Jacek Dach
Magdalena Szczech
Małgorzata Jędryczka
Agnieszka A. Pilarska
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Abstract

The aim of the research was to assess the microbiological (number of heterotrophic bacteria, actinobacteria and moulds) and biochemical (urease and acid phosphatase activity) state of peat with the admixture of composts produced from sewage sludge. An additional aim of the research was to demonstrate the influence of those substrates on the morphological traits of scarlet sage (height, number and length of shoots, number of buds and inflorescences, greenness index (SPAD)). Composts produced from sewage sludge, wheat, maize and lupine straw were mixed with peat, where their percentage varied from 25% to 75%.

The substrate which included the composts applied in the experiment had a higher number of heterotrophic bacteria and a higher acid phosphatase activity level than the control substrate (peat). The multiplication of moulds and actinobacteria was more intensive than in the peat only in the combinations with K3 (sewage sludge 50%+sawdust 20%+ lupine straw 30%) and K4 (sewage sludge 50%+sawdust 20%+fresh maize straw 30%) composts, whereas the highest urease activity level was observed in the soils produced from K1 (sewage sludge 50%+sawdust 20%+white straw 30%) compost.

The most optimal development of plants was observed in the substrate with compost produced from wheat straw. Composts produced from municipal sewage sludge were found to be suitable for growing scarlet sage. However, their effect depends on the percentage of high peat in the substrate.

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Authors and Affiliations

Agnieszka Wolna-Maruwka
Klaudia Borowiak
Agnieszka Mocek-Płóciniak
Katarzyna Głuchowska
Anita Schroeter-Zakrzewska
Alicja Niewiadomska
Justyna Starzyk
Jacek Dach

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