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Abstract

Current study was designed to investigate the protective effects of royal jelly on Flunixin me- glumine (FM)-induced spermiotoxicity related to sperm concentration, abnormal spermatozoa count and histopathological changes in mice testis. The subjects were divided into five groups according to FM and/or royal jelly intake: Control group; group 1, FM alone (25 mg/kg, im); group 2, combination of FM (25 mg/kg, im) and royal jelly (200 mg/kg, oral); group 3, FM alone (50 mg/kg, im); and group 4, combination of FM (50 mg/kg, im) and royal jelly (200 mg/kg, oral). The animals were fed once daily for 15 days and they were sacrificed last day. Epididymal sperm concentration and abnormal spermatozoa count were noted. Testicular histological findings were evaluated. On purpose, organization of each animal was graded according to Johnsen’s scoring to assess the spermatogenesis relying on seminiferous tubule cross-section scores. Comparing to controls, FM administration caused a decrease in sperm concentration (p<0.05), an increase in total abnormal spermatozoa rates (p<0.05) and more degenerative changes in testes in mice.

Royal jelly supplementation ameliorated both sperm concentration and abnormal spermato- zoa (p<0.05) comparing to the control group. In conclusion, we suggested that royal jelly might have protective effects in the FM-induced reductions in epididymal sperm concentration and in- crease in abnormal spermatozoa rate.

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Authors and Affiliations

F. Temamoğulları
F. Aral
R. Yılmaz
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Abstract

Nickel damages the male reproductive system. We investigated the beneficial effects of silibinin which has metal-chelating and antioxidant properties over nickel toxicity. Both antioxidative effects in testes and overall effects related to sperm motility, membrane and acrosome integrity of orally administered Silibinin were evaluated against the harmful effects of 30 day of intraperitoneal nickel sulfate (5 mg/kg/day) administration in rats. Male rats were randomized into control (Group1; n=6) and three experimental groups (n=6, each): Group2 Nickel sulfate (5 mg/kg/day), Group3 Silibinin (150 mg/kg/day), and Group 4 Nickel sulfate (5 mg/kg/day) + Silibinin (150 mg/kg/day). We found higher sperm motility, viable sperm and total sperm count in Groups 3 and 4 than the Group 2 treatment groups and the percentage of abnormal spermatozoa was similar in both groups (Groups 2 and 4). Increased apoptosis, activation of caspase3, 8, 9 and TUNEL were detected in Group 2. However, activation of caspase3, 8, 9 and TUNEL was reduced in Group 4. The protective effects of silibinin were demonstrated on histopathologic findings and some sperm parameters (sperm motility percentage, viable spermatozoa, sperm count, and abnormal spermatozoa percentage) in rats exposed to nickel.
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Authors and Affiliations

F. Temamogullari
1
A. Atessahin
2
C. Cebi Sen
3
N. Yumusak
4
M.S. Dogru
5

  1. Department of Pharmacology and Toxicology, University of Harran, Faculty of Veterinary Medicine, 63200 Şanlıurfa, Turkey
  2. Department of Pharmacology and Toxicology, University of Fırat, Faculty of Veterinary Medicine, 23119 Elazığ, Turkey
  3. Department of Reproduction and Artificial Insemination, University of Harran, Faculty of Veterinary Medicine, 63200 Şanlıurfa, Turkey
  4. Department of Pathology, University of Harran, Faculty of Veterinary Medicine, 63200 Şanlıurfa, Turkey
  5. Department of Pharmacology and Toxicology, University of Aksaray, Faculty of Veterinary Medicine, 6800 Aksaray, Turkey

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