The current passed by the stator coil of the permanent magnet synchronous motor (PMSM) provides rotating magnetic field, and the number of turns will directly affect the performance of PMSM. In order to analyze its influence on the PMSM performance, a 3 kW, 1500 r/min PMSM is taken as an example, and the 2D transient electromagnetic field model is established. The correctness of the model is verified by comparing the experimental data and calculated data. Firstly, the finite element method (FEM) is used to calculate the electromagnetic field of the PMSM. The performance parameters of the PMSM are obtained. On this basis, the influence of the number of turns on PMSM performance is quantitatively analyzed, including current, no-load back electromotive force (EMF), overload capacity and torque. In addition, the influence of the number of turns on eddy current loss is further studied, and its variation rule is obtained, and the variation mechanism of eddy current loss is revealed. Finally, the temperature field of the PMSM is analyzed by the coupling method of electromagnetic field and temperature field, and the temperature rise law of PMSM is obtained. The analysis of this paper provides reference and practical value for the optimization design of PMSM.

An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its expression in CHO-K1 cells and the inhibition effect of Congjiang pig IFN-λ1 on PRRSV proliferation was verified. The eukaryotic expression plasmid pEGFP-PoIFN-λ1 was constructed from the pig IFN-λ1 gene fragment and transfected into CHO-K1 cells. Expression was detected by fluorescence microscopy and Western blotting. The influence on the proliferation of PRRSV was assessed. The results of the study showed that the recombinant plasmid pEGFP-PoIFN-λ1 was constructed correctly. After transfection, green fluorescent signal was detected in CHO-K1 cells by fluorescence microscopy. Western blot analysis revealed that in cells at different time periods after transfection, porcine IFN-λ1 was expressed, with the highest expression observed 36 h after transfection. The antiviral activity of the supernatant after 36 h of transfection was determined by the micro cytopathic inhibition method, and the biological activity was 2.1×103 U/mL. Quantitative PCR was used to detect the proliferation of PRRSV, and the results showed that Congjiang pigs IFN-λ1 significantly inhibited the mRNA expression of PRRSV and viral proliferation in a dose- and time-dependent manner. This study established a Congjiang pig IFN-λ1 eukaryotic expression system, and the quantitative PCR method showed that it has a significant inhibitory effect on the proliferation of PRRSV, which lays a foundation for the future production of antiviral drugs and clinical application.