Biological activity of 6 fungicides in the inhibition of Phytophthora ramorum sporulation and development of blight on rhododendron leaves and stems were evaluated. All tested compounds at dose 8 μg o fa.i./cm3 already inhibited zoosporangia formation at least in 73%. On leaf petioles and leaf disks, taken from rhododendron one week after treatment with fungicides, formation of chlamydospores was especially suppressed by fenamidone + phosetyl Al and oxadixyl + mancozeb whereas development o fspores was not inhibited by cymoxanil + famoxate. All tested compounds significantly inhibited the development and spread of twig blight on rhododendron. However, furalaxyl, applied as spraying of plants 48 hrs before or after inoculation of leaves and stems by P. ramorum was the most effective.
Phytophthora citricola Sawada was detected from 3 water pools situated in 2 container- grown nurseries. The highest number of spots on rhododendron leaves were observed in June whereas the lowest in October. The use of water for plant sprinkling caused browning, yellowing of shoots and root rot of Buxus sempervirens, and blight of shoot tips of Thuja occidentalis and Rhododendron sp. The disease symptoms were observed already in June and the disease developed till the first decade of October. Losses caused by the species varied from 9 to 56%.
Phytophthora cambivora was isolated from the bark lesions of two 10- and 15-year-old of analysed alder trees. Additionally, Botrytis cinerea, 3 Fusarium species, Mucor spp., P. alni and Trichoderma spp. were recovered from diseased tissues. Isolates of P. cambivora from six plant species, used for inoculation of alder seedlings and plant parts, cause dthe development of necrosis. Isolate from Chamaecyparis lawsoniana was the weakest pathogen whereas those from Abies alba, Acer pennsylvanicum and Alnus glutinosa were the strongest.
Phytophthora cryptogea was isolated from diseased stem base of Aquilegia discolor and rotted leaves of Saxifraga and Sempervivum spp. Additionally, Fusarium species and Botrytis cinerea were frequently isolated from diseased parts of plants. Most of Sempervivum species and cultivars except S. soculiferum were colonized in laboratory conditions by P. cryptogea. The isolates from Alstroemeria aurantiaca, Gerbera jamesonii, Saxifraga arendsii, S. paniculata, Sempervivum arachnoideum colonised houseleek leaves with the fastest spread of necrosis on plant parts inoculated with cultures from Gerbera and S. arendsii. The isolate from S. arendsii colonized 5 species and cultivars of saxifrage as well as Iberis sempervivum, Lavendula angustifolia, Sempervivum sobuliferum and Vinca minor with the slowest development on periwinkle. In laboratory trials isolate of P. cryptogea from houseleek grew on PDA and colonized leaves of that plant at temperature ranging from about 10 to 32.5°C with optimum at 20–30°C.
Phytophthora citricola was isolated from diseased seedlings of European beech and Silver fir taken from the most of surveyed nurseries. Fusarium species, Pythium ultimum and Rhizoctonia solani were also found in diseased plant tissues.Isoates of P. citricola fro mboth plants and additionally from heather and rhododendron colonised leaf blades, needles and stem parts of beech and fir. In greenhouse trials on inoculated 1-year-old seedlings necrosis spread about 2 mm/24 hr on beech stems whereas on fir about 1.5 mm/24 hr.
Phytophthora citricola constituted about 70% of all Phytophthora isolates recovered from rhododendron leaves used as baits for detection of that group of organisms in water. The species was found in 4 rivers, 2 hardy nursery water reservoirs and nursery drainage canal from May to October, 2006. Analysis of spots’ number on rhododendron leaf baits as a measure of P. citricola density showed that place of holding baits had a significant influence on the species occurrence. Significantly more spots, especially in July survey, were observed on baits held in Skierniewka and Zwierzynka rivers swimming through agricultural and forest area than in Ner, the river of horticultural area. Significantly more spots/rhododendron leaves were observed when they were held in rivers downstream of nursery and in the middle of hardy nursery borders. In nursery water containers and drainage canal higher Phytophthora density was recorded in August than in other periods of surveying. Using water from reservoir for sprinkling of Picea omorika nursery trees caused the development of tip blight and from diseased twigs P. citricola was isolated.
Research over a three year period indicated that P. ramorum occurred rarely in Poland on Rhododendron spp., in spite of established monitoring in nurseries, trade stands, forest and water from early spring to late autumn each year. The pathogen was not found in forests on Vaccinium vitisidaea, Calluna vulgaris, Fagus sylvatica and Quercus rubra, proving its limited spread. The species was detected, however, from 2 rivers. P. citricola was isolated from most of surveyed plants. Besides this P. cactorum, P. cinnamomi, P.citrophthora and P. nicotianae var. nicotianae were isolated from diseased plants. Additionally Pestalotia sydowiana, species of Fusarium, Botrytis cinerea and Trichoderma were often found in diseased plant tissues. Laboratory and glasshouse research showed slight differences in colonization of plants by P. ramorum and P. citricola. However, taking into account the range of host plants, and frequency of pathogen occurrence in infected plant material and water, it became clear that P. citricola poses a much greater danger than P. ramorum to the natural environment in Poland.
From Hedera helix and Epipremnum aureum showing necrosis of shoot base spread upwards and on leaves Phytophthora tropicalil was isolated. The species was obtained from ⅞of Hedera and ¾ of Epipremnum diseased shoot and root parts. Additionally, Botrytis cinerea, Fusarium avenaceum and Rhizoctonia solani was recovered from some of affected plants. The chosen 2 isolates colonised petioles and leaf blades of both host plants. P. tropicalis caused necrosis of leaves of 11 tested cultivars of H. helix and 13 other pot plant species and seedlings of tomato. The fastest spread of necrosis was observed on leaves of Peperomia magnoliaefolia, Pelargonium zonale and Phalaenopsis x hybridum. The development of disease was observed at temperatures ranged from 10 to 32.5°C with optimum 30°C.
Genotypic differentiation among 10 isolates of Phytophthora cinnamomi Rands and 24 isolates of Phytophthora citricola Sawada from 12 different plant species grown in Polish ornamental nurseries was determined. DNA was extracted from pure pathogen cultures and amplified by the PCR technique using ISSR and RAPD primers. 9 primers were used to amplify P. cinnamomi and 8 to amplify P. citricola DNA. The analyzed amplification products were between 300 and 2300 bp. The genotypical differentiation was from 17 to 35% in P. cinnamomi and from 10 to 60% in P. citricola. Isolates from host plants of the same family showed, with some exceptions, similar levels of differentiation.
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