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Abstract

I n t r o d u c t i o n: Induction of labor is an intervention in the obstetrics, which aim is to achieve cervical ripening and stimulate contractions of uterus before beginning of labor. The purpose of our study was to evaluate efficacy of combinations of vaginal misoprostol, intracervical dinoprostone and Foley catheter at term with regard to mode of delivery and rate of emergency C-sections due to birth asphyxia.

Ma t e r i a l a n d Me t h o d s: 403 singleton pregnant women, who underwent pharmacological labor induction at term, were reviewed. Patients were divided into 2 main cohorts due to beginning of induction algorithm: vaginal misoprostol (66) or intracervical dinoprostone (337) consisting of 3 subgroups — PGE2 alone (184), PGE2+Foley catheter (125), PGE2+Foley catheter+PGE1 (28).

R e s u l t s: Comparison of maternal age, presence of cervical dilation and parity revealed no major differences between cohorts. Eff ectiveness of labor induction with misoprostol, dinoprostone and dinoprostone followed by Foley catheter were respectively 90.9%, 51.3%, and 82.8%. Addition of PGE1 was effective in 83% of patients with negative response to PGE2 followed by Foley catheter. There was no statistically significant diff erence in rate of C-sections between dinoprostone and misoprostol cohorts, C-section due to birth asphyxia were insignificantly more frequent in PGE1 than in PGE2 cohort. Efficacy in the subgroup administered only dinoprostone was significantly higher in 40th than in 41th (p = 0.016).

C o n c l u s i o n s: Intracervical dinoprostone seems to be safer, but less effective in labor induction than vaginal misoprostol. Following PGE2 by other methods increased efficacy of induction in this cohort.

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Authors and Affiliations

Anna Modrzyńska
Małgorzata Radoń-Pokracka
Magdalena Płonka
Beata Adrianowicz
Gabriela Wilczyńska
Magdalena Nowak
Hubert Huras
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Abstract

Megasporogenesis and female gametophyte development were investigated in ovules of the everbearing strawberry Fragaria x ananassa Duch. cv. Selva. Observations of thin sections revealed that ovule development starts from the formation of a nucellus and coincides in time with the beginning of receptacle overgrowth. The most characteristic feature during nucellus differentiation is the formation of a multicellular archesporium, beginning from at least two cells. Analysis of female gametophyte development indicated that in addition to the meiotic mode, female gametophytes develop by an apomeiotic mode of Antennaria type. Asynchronous development of female gametophytes of different origin occurs. The mature, eight-nucleate, seven-celled female gametophyte of meiotic origin is cylindrical and slightly curved. It occupies the central part of the nucellus. The egg apparatus, consisting of an egg cell and two synergids, is formed in the micropylar part of the female gametophyte; the opposite chalazal pole is occupied by antipodal cells. Besides the ovule in which only one seven-celled female gametophyte finally develops, ovules with a different number of cells were observed to initiate female gametophyte development. Some ovules contain a nucellus with a tetrad of linearly arranged megaspores surrounded by enlarged cells, each of which has the potential to develop into an apomictic female gametophyte. After degeneration of some post-meiotic cells or developmentally advanced female gametophytes, some of the chalazal cells initiated female gametophyte development.

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Authors and Affiliations

Ewa Dziadczyk
Magdalena Nowak
Marcin Domaciuk
Ewa Szczuka
Józef Bednara
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Abstract

This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fluorescens SBW25 and Burkholderia xenovorans LB400 species were dominant.

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Authors and Affiliations

Anna Ząbkiewicz
Magdalena Myga-Nowak
Katarzyna Bandurska
Justyna Paczyńska
Anna Szybecka
Piotr Krupa

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