Abstract
A novel avian orthoreovirus (N-ARV) variant characterized with obvious arthritis and synovial inflammation, was isolated from Shandong, China in May 2016. It caused chicken poor growth and enormous economic losses to the poultry industry of China. However, there are few effective methods for detecting the antibody levels of N-ARV. In this study, a viral structural protein σC was expressed using the prokaryotic expression vector pET32a (+). The target protein was obtained by inducing for 6 hours at an IPTG concentration of 0.6mM. The optimal dilution of the coating antigen and serum antibody were determined to be 1000 fold and 10 fold respectively. A specificity test showed that there was no positive reactivity between N-ARV and other pathogens, and when the positive serum was diluted 100 times detection results were still checkable. The repeatability of this method was determined by the inter assay and intra assay tests with variability ranging from 4.85% to 7.93%. In conclusion, this indirect enzyme linked immunosorbent assay (ELISA) will be useful for large-scale serological surveys and monitoring antibody levels in N-ARV infection.
Go to article
Bibliography
Adair BM, Burns K, McKillop ER (1987) Serological studies with reoviruses in chickens, turkeys and ducks. Comp Pathol 97:495-501.
Ayalew LE, Gupta A, Fricke J, Ahmed KA, Popowich S, Lockerbie B (2017) Phenotypic, genotypic and antigenic characterization of emerging avian reoviruses isolated from clinical cases of arthritis in broilers in saskatchewan. Scientific Reports 7: 3565.
Benavente J, Martinezcostas J (2007) Avian reovirus: structure and biology. Virus Res 123:105–119. Bodelón G, Labrada L, Martønez-Costas J (2001) The avian reovirus genome segment S1 is a functionally tricistronic gene that expresses One structural and two nonstructural proteins in infected cells. Virology 290:181-191.
Caterina, KM, Frasca S, Girshick T, Khan M (2004) Development of a multiplex pcr for detection of avian adenovirus, avian reovirus, infectious bursal disease virus, and chicken anemia virus. Molecular and Cellular Probes 18: 293-298.
Curtis PE, Al-Mufarrej SI, Jones RC, Morris J, Sutton PM (1992) Tenosynovitis in young pheasants associated with reovirus, staphylococci and environmental factors. Veterinary Record 131: 293.
Dandár E, Bálint Á, Kecskeméti S (2013) Detection and characterization of a divergent avian reovirus strain from a broiler chicken with central nervous system disease. Arch Virol. 158: 2583-2588.
Dutta SK , Pomeroy BS (1967) Isolation and characterization of an enterovirus from baby chicks having an enteric infection II. Physical and chemical characteristics and ultrastructure. Avian Dis 11: 9-15.
Fahey JE , Crawley JF (1954) Studies On Chronic Respiratory Disease Of Chickens II. Isolation of A Virus. Can J Comp Med Vet Sci 18: 13-21.
Guo K, Dormitorio TV, Ou SC Giambrone JJ (2011) Development of TaqMan real-time RT-PCR for detection of avian reoviruses. Virol Methods 177: 75-79.
Heggen-Peay CL, Qureshi MA, Edens FW (2002) Isolation of a reovirus from poult enteritis and mortality syndrome and its pathogenicity in turkey poults. Avian Dis 100: 102-105
Howell SH, Walker LL ( 1972) Synthesis of DNA in toluenetreated Chlamydomonas reinhardi (DNA replication-chloroplast DNA-cell cycle-electron microscopy). Proceedings of the National Academy of Sciences of the United States of America 69: 490-494.
Ide PR, Dewitt W (1979) Serological incidence of avian reovirus infection in broiler-breeders and progeny in Nova Scotia. Canadian Veterinary Journal La Revue Vétérinaire Canadienne 20: 348.
Jones RC, Kibenge F (1984) Reovirus-induced tenosynovitis in chickens: The effect of breed. Avian Pathol 13: 511-528.
Jones RC, Savage CE (1987) Effects of experimental immunosuppression on reovirus-induced tenosynovitis in light- -hybrid chickens. Avian Pathol 14: 51-58.
Lin FQ, Qi-Lin HU, Chen SY (2007) Diagnosis of Muscovy Duck Reovirus Disease by Semi-Nested RT-PCR. Journal of South China Agricultural University 16: 61-68.
Liu HJ, Giambrone JJ, Nielsen BL (1997) Molecular characterization of avian reoviruses using nested PCR and nucleotide sequence analysis. J Virol Methods 65: 159-167.
Liu Y, Wan W, Gao D ( 2016) Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China. Emerg Microbes Infect 5: 117.
Marks M , Marks JL (2016) Viral arthritis. Clinical Medicine 1: 16-19. Mor SK, Sharafeldin TA, Porter RE (2014) Molecular characterization of L class genome segments of a newly isolated turkey arthritis reovirus. Infect Genet Evol 27: 193-201.
Mor SK, Sharafeldin TA, Porter RE (2013) Isolation and characterization of a turkey arthritis reovirus. Avian Dis 57: 97-103.
Noh JY, Lee DH, Lim TH, Lee J H, Day JM, Song CS (2018) Isolation and genomic characterization of a novel avian orthoreovirus strain in Korea, Arch Virol 163: 1-10.
Victor PT, Darko M, Tom I, Frank V, Faizal A (2018) Molecular characterization of emerging avian reovirus variants isolated from viral arthritis cases in western canada 2012-2017 based on partial sigma (σ)c gene. Virology 522: 138-146.
Palya V, Glávits R, Dobos-Kovács M, É Ivanics, Nagy, E, K Bányai (2003) Reovirus identified as cause of disease in young geese. Avian Pathol 32: 129-138.
Xie L, Xie Z, Wang S, Deng X, Xie Z (2020) Study of the activation of the pi3k/akt pathway by the motif of σa and σns proteins of avian reovirus. Innate Immunity 26: 4.
Rendón-Anaya M, Montero-Vargas JM, Saburido-Álvarez S, Vlasova A, Herrera-Estrella A (2017) Genomic history of the origin and domestication of common bean unveils its closest sister species. Genome Biology 18: 60.
Sharafeldin TA, Mor SK, Bekele AZ, Verma H, Noll SL, Goyal SM (2015) Experimentally induced lameness in turkeys inoculated with a newly emergent turkey reovirus. Veterinary Research 46: 11.
Shien JH, Yin HS , Lee LH (2000) An enzyme-linked immunosorbent assay for the detection of antibody to avian reovirus by using protein σ B as the coating antigen. Res Vet Sci. 65: 60
Wen L, Hwh B, Ming H, Long H, Hjlb C (2004) Avian reovirus σc protein induces apoptosis in cultured cells. Virology 321: 65-74.
Tang Y, Lu H (2015) Genomic characterization of a broiler reovirus field strain detected in pennsylvania. Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases 31: 177-182.
Tang Y, Lin L, Sebastian A, Lu H (2016) Detection and characterization of two co-infection variant strains of avian orthoreovirus (ARV) in young layer chickens using next-generation sequencing (NGS). Sci Rep 6: 24519.
Vasserman Y, Eliahoo D, Hemsani E (2004) The influence of reovirus sigma C protein diversity on vaccination efficiency. Avian Dis 47: 28-30
Vindevogel H, Meulemans G, Pastoret PP (1982) Reovirus infection in the pigeon. Ann Rech Vet Rinaires Ann Vet Res 13: 149.
Yun T, Chen H, Yu B (2015) Development and application of an indirect ELISA for the detection of antibodies to novel duck reovirus. Journal of Virological Methods 220: 55-59.
Go to article
en
pl
