The deterrent effect of limonene enentiomers and limonene derivative: ( + )-(1S,4S,6S)-4-(1-methylethenyl)-9-oxabicyclo[ 4.3.0]nonan-8-one, on Myzus persicae was investigated. Only S-enantiomers of limonene and its derivative showed feeding deterrent properties. S-limonene inhibited phloem sap ingestion and reduced the number of phloem sap ingestion phases during aphid stylet penetration of plant tissues. Aphids spent twice less time on leaves painted with ( + )-1S,4S,6S-4-(1-methylethenyl)-9-oxabicyclo[ 4.3.0]nonan-8-one than on control and S-limonene-treated leaves and the probes were shorter than 2 minutes on these leaves. Our studies confirmed that the chiral centre configuration of the lactones was important in expression of feeding deterrent activity.