The temperature dependence of the particle size distribution (PSD) of the magnetic fluid with an additional biocompatible dextran layer was studied using a ultrasonic method. The measurements of the ultrasound velocity and attenuation were carried out as a function of the volume concentration of magnetite particles at temperatures ranging from 15°C to 40°C. In order to extract the PSD from ultrasonic measurements, the theoretical model of Vinogradov-Isakovich was used. The extraction of PSD from the ultrasonic data requires also the measurements the density and viscosity of the ferrofluid samples. The calculated PSD of the magnetic fluid with an additional biocompatible layer shows a greater thermal stability than that of a magnetic fluid with a single surfactant layer.