In this paper we present the first identification of the Tomato clack ring virus isolated from zucchini with mosaic and deformation of leaves in Poland. Immunosorbent electron microscopy, ELISA test and IC-RT-PCR confirmed the identification of TBRV. RNA extracted from purified virus (size about 7.4 kb and 4.6 kb) was characteristic to this virus.

From the naturally infected cucumber plane spherical virus was isolated that mainly on basis of its serological properties has been identified as Tomato black ring virus (TBRV). Using antiserum against TBRV-ED for the specific crapping of virus followed by PCR test (immunocapture-RT-PCR) allowed co distinguish TBRV from related viruses, especially Beet ringspot virus (BRSV). Presence of as many as rwo satellite RNAs should be found as a unique feature of the cucumber isolace.