Applications of morphological filters for two-process profiles were analysed. Dilation, closing and alternating sequential (closing +  opening) filters were used with a circle (disk) as a structuring element. An original method of a disk radius selection was elaborated for two-process profiles. This procedure was applied for many simulated and measured profiles. Behaviors of morphological filters were compared with those of double Gaussian (Rk) filter. Robust filter was also taken into consideration. In calculation, TalyMap software was used. The proposed procedure was found to be very useful. It was developed for 2D profiles but it can be easily extended for an areal (3D) surface topography filtering. From among morphological filters, the alternate sequential filter is suggested.

The two aims of this study were to obtain stable thyme-oil loaded nanoemulsions using the statistical design of experiment method (DOE) and to confirm their antimicrobial and disinfecting properties. Thyme oil was used as the oil phase, ECO Tween ® 80 acted as an emulsifier, and the rest of the formulation was deionized water. Ultrasonication was chosen as the method of obtaining the nanoemulsions. It was checked whether the input parameters (oil concentration, emulsifier concentration, amplitude, and sonication time) had a significant impact on the output parameters (nanoemulsion particle size, polydispersity index, viscosity, and stability over time). For the formulations selected on the basis of the statistical data analysis, the values of minimum inhibitory concentrations (MIC) and minimum biocidal concentrations (MBC/MFC) were determined in relation to 10 bacterial strains and 10 strains fungi (filamentous fungi, yeast-like fungi). The results obtained from the statistical analysis showed that the optimal concentration of the thyme oil in nanoemulsion should amount up to 2%. Biological studies proved that the obtained formulation had stronger antibacterial and antifungal activity compared to pure oil. Moreover, it was shown that the nanoemulsion caused the required for disinfectants reduction of > 5 log of bacterial strains ( S. aureus, P. aeruginosa) and > 4 log of fungal strains ( C. albicans) after 30 minutes.

The process of obtaining alginate microspheres (AMs) by emulsification method was optimized by applying statistical analysis software. Ten batches of microspheres were prepared using the fractional plan 3 ^(K-p). AMs were obtained with two different methods: an ultrasonic homogenization (UH) process and a rotor-stator mechanical homogenization (MH). The amount of a cross-linking agent (CaCl ₂), calcium chloride rate addition, and the sonication amplitude (UH) or the speed of rotor rotation (MH) were selected as formulation variables. All the batches were evaluated in terms of stability and size of the alginate microspheres. Approximation profiles were developed. As a result of the conducted research, stable alginate microspheres with sizes ranging from 10 to 30 micrometres were obtained. The obtained results showed that the quality of AMs was mainly affected by the concentration and the rate of calcium chloride addition into the system. Therefore, the role of calcium ions in the mechanisms of shell structuring was discussed. Lactobacillus casei bacteria were encapsulated into the batches found to be optimum. The high encapsulation efficiency (EE) of the bacteria (72-94%) depending on the form) and their viability over time were obtained. The model developed in the study can be effectively utilized to achieve the AMs formulations.