The results of the study of the macrophytes of the rivers Turia, Vyzhivka and Tsyr, the right-bank tributaries of the upper reaches of the Pripet River, are presented. The species composition of aquatic and coastal aquatic vascular plants was investigated during the vegetation seasons of 2018 and 2019 at 9 observation points located along the rivers from the source to the mouth. The most numerous species composition, 57 species, was found in the Turia River, 36 in the Vyzhivka River, and 28 species were identified in the Tsyr River. The macrophyte index for rivers (Pol.: makrofitowy indeks rzeczny – MIR) was determined from the results, and the ecological state of the rivers was assessed according to the methodology of the macrophyte assessment of rivers (Pol.: makrofitowa metoda oceny rzek – MMOR). The investigation showed that water quality in the Turia River on sites No. 1, 3 and 4 belongs to class III, satisfactory category. The surface water quality on site No. 2 of the Turia River belongs to class II, a good category. The water quality in the Vyzhivka River on all test sites belongs to class II, a good category, which testifies to favourable ecological conditions for the development of higher aquatic plants along the whole course. The water quality in the Tsyr River on test site No. 8 (Kamin-Kashyrskyi, upper course) corresponds to class II, good category. On test site No. 9 (middle course), the quality of surface waters of the Tsyr River worsens to the class III, satisfactory category.