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Abstract

This article shows acoustic measurements from Svalbard archipelago in the Arctic, located in the Arctic Ocean. The aim of the research was to show the Svalbard soundscape as well as to record and analyse the spatial-temporal dynamics of the acoustic environment, the human impact on the soundscape and to collect baseline data for future comparative research. Svalbard is interesting for many science disciplines because it has an arctic climate and, at the same time, it is relatively easily accessible. Climatologists, geologists, glaciologists, biologists and even anthropologists could find interesting themes to investigate here. Additionally, the soundscape of Spitsbergen is worthy of detailed examination. This paper presents comparative analysis of the soundscape of various spots near Longyearbyen in Management Area 10. The soundscape analysis of selected valleys shows the strong influence of human activity on the soundscape as well as the variability and characteristic features of the natural Arctic soundscape.

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Authors and Affiliations

Dorota Czopek
Paweł Małecki
Janusz Piechowicz
Jerzy Wiciak
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Abstract

The aim of this work was to evaluate the relative gene expression levels of the cytokines IL- 1B, IL-8, IL-12, IFN-γ, IL-4, IL-10 and TGF-β in somatic milk cells of French Alpine breed, anestrous goats that were experimentally infected in the left mammary gland with Staphylococcus chromogenes during the lactation peak. Milk samples were obtained from both glands for 21 consecutive days post infection. Total RNA was extracted, and real-time PCR was conducted using primers specific to each cytokine. The relative RNA expression of the evaluated cytokines was determined by the comparative method 2-ΔΔCT, using milk from the right gland of the goats as a reference (control) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous control. According to the Wilcoxon test results, IL-1B and IL-12 expression levels showed significant differences compared to those in the control group (p<0.05) from 24 hours post infection until the end of lactation; on day three, IL1β, IL8, IL12 and TGF-β had a statistically significant change in expression with respect to those in the control group (p<0.05); closer to the end of the lactation period, there is no overexpression of the anti-inflammatory interleukins (IL-4 and TGF-β) which may reflect the effort of the host immune system to eradicate the microorganism from the mammary gland.

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Authors and Affiliations

R.A. Ruiz-Romero
D. Martínez-Gómez
R.A. Cervantes-Olivares
E. Díaz-Aparicio
A.E. Ducoing-Watty

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