This research analysed the availability of phytoplankton and the growth rate of Vannamei shrimp in relation to water quality changes. The research was carried out in February–March 2021 for a half cycle of shrimp cultivation in two ponds of the Brackish Water Fish Culture Probolinggo Laboratory in Probolinggo, East Java, Indonesia. The research used a descriptive method and included a survey. Sampling was made every two weeks for two months. Nine parameters were measured and ten shrimps were taken for a specific growth rate ( SGR) measurement once per sampling. Data were analysed using the principal component analysis (PCA) and canonical correspondence analysis (CCA). Secondary data of water quality were added for the PCA. The results show that the phytoplankton found in the first pond consisted of Chlorophyta, Chrysophyta, and Cyanophyta, whereas the phytoplankton in the other pond included Chlorophyta, Chrysophyta, Cyanophyta, and Dinophyta. The abundance of phytoplankton ranged from 12–80∙10 ³ cell∙cm ^–3, which indicated eutrophic waters. The PCA demonstrated that pH, nitrate, and total organic matter (TOM) significantly influenced phytoplankton abundance in the pond. In addition, water quality parameters, such as temperature, transparency, salinity, nitrite and phosphate levels, were tolerable in both ponds for the growth of shrimps. However, the level of pH was lower than the aquaculture quality standard, whereas those of nitrate, ammonia, and TOM were higher. The growth rate of Vannamei shrimp increased by 0.76–7.34%∙day ^–1.

In this work, thermo-mechanically treated 42CrMo steel was subjected to cryogenic treatment conducted by means of orthogonal design method, followed by low-temperature tempering to investigate the effect of different parameters of cryogenic treatment on wear resistance of 42CrMo steel and to optimize parameters of cryogenic treatment for improving wear resistance. The results of hardness test and wear test show that cryogenic treatment significantly improves wear resistance with marginal changes in coefficient of friction and hardness. Specifically, cryogenic temperature has the largest impact on wear resistance of 42CrMo steel, holding time has medium impact, and the parameter of treatment cycles has the least impact. The optimum parameters of cryogenic treatment are −196°C for 12 hours with one cycle for improving wear resistance. The results of scanning electron microscopy (SEM) and X-ray diffractometry (XRD) analysis indicate that marginal changes in hardness and coefficient of friction may be owing to little amount of transformation of retained austenite, and the significant influence of cryogenic treatment on improving wear resistance of 42CrMo steel can be mainly attributed to segregation of carbon atoms promoted by cryogenic treatment resulting in more precipitation of carbides in subsequent tempering.