Onion yellow dwarf virus (OYDV), an aphid-borne potyvirus is one of the major viral pathogens of garlic causing significant yield losses worldwide. It is found almost everywhere in the world where Allium species is grown. The aim of this study was to test the presence of OYDV infection in garlic from Ethiopia. The presence of the virus was tested by Reverse transcription polymerase chain reaction (RT-PCR). The direct sequencing of the PCR product produced a sequence of 296 bp. Sequence analysis showed 89.27% sequence homology with an isolate from Australia (HQ258894) and 89.29% with an isolate from Spain (JX429964). A phylogenetic tree constructed with MEGA 7.0 revealed high levels of homology with various isolates of OYDV from all over the world and thus further confirmed the identity of the virus.

This article deals with the effects of electrical discharge machining (EDM) on the chemical composition and microstructure of cast Alnico alloys, i.e., iron-based alloys composed of aluminum, nickel and cobalt. The experiments focused on determining the chemical composition of the surface layer before and after the EDM process. The microstructure of the material altered by the EDM was also examined. The study included measurement of the thickness of the white layer characteristic of EDM. It is evident that low values of the surface roughness parameters can be obtained by correctly selecting the EDM process parameters. The average surface roughness reported in the experiments was 1 μm. The surface roughness measurements were conducted with a Talysurf CCI lite non-contact profiler. The metrological results also indicate that lower surface roughness can be obtained at small discharge energies.