This paper aims to enhance the productivity of a chilled beef production line by comparing two techniques; standard time calculation and simulation. The best improvement method was obtained using the work-study principle, a network diagram, and bottleneck identification. Two methods for improvement are proposed based on the ECRS, the Theory of Constraint (TOC), and line balancing concepts. A simulation model is developed to mimic the actual production line. The simulation results are verified, validated, and compared. Some workstations were combined, and the allocation of the workers was arranged. The present production line efficiency was 46.21%, which increased to 67.09% and 79.71% from the suggested methods. It showed that using the standard time calculation gives a different result from the simulation. In summary, the simulation model along with the application of TOC and ECRS, provides accurate information and improves overall productivity.