In the current study, twenty lambs, aged 4 months, half male and half female, were classified into four groups, with five in each group. The experimental three groups of lambs were given intravenous (IV), intramuscular (IM) and subcutaneous (SC) administrations of recombinant ovine interferon-τ (roIFN-τ). The fourth group (normal control) of lambs was given normal saline injections in the same way. After administrations, blood samples were collected from the tested animals at different time points post injection, and the serum titers of roIFN-τ were measured using cytopathic effect (CPE) inhibition bioassay. The results of calculating pharmacokinetic (PK) parameters using DAS software showed that the PK characteristics of roIFN-τ through IV injection conformed to the two-compartment open model, whose half-life of distribution phases (T1/2α) was 0.33±0.034 h and the elimination half-life(T1/2β) was 5.01±0.24 h. However, the PK features of IM injection and SC injection of roIFN-τ conformed to the one compartment open model, whose Tmax were 3.11±0.26 h and 4.83±0.43 h, respectively, together with an elimination half life(T1/2β) of 9.11±0.76 h and 7. 43±0.58 h, and an absorption half-life (T1/2k(a)) of 1.13±0.31 h and 1.85±0.40 h, respectively. The bioavailability of roIFN-τ after IM administration reaches 73.57%, which is greater than that of SC administration (53.43%). These results indicate that the drug administration effect can be preferably obtained following a single dose IM administration of the roIFN-τ aqueous preparation. This study will facilitate the clinical application of roIFN-τ as a potential antiviral agent in future work.
Kinetic resolution of (R)- and (S)-mandelic acid by its transesterification with vinyl acetate catalysed by Burholderia cepacia lipase has been studied. The influence of the initial substrate concentration on the kinetics of process has been investigated. A modified ping-pong bi-bi model of enzymatic transesterification of (S)-mandelic acid including substrate inhibition has been developed. The values of kinetic parameters of the model have been estimated. We have shown that the inhibition effect revealed over a certain threshold limit value of the initial concentration of substrate.
Point of present exploration was to figure out the anticorrosion activity of Acacia Cyanophylla (Saligna leaves) extract on the corrosion of mild steel in dilute sulfuric acid medium, using weight loss measurements and electrochemical impedance spectroscopy. The result of the study revealed that the extract act as a potent inhibitor on mild steel in acid medium. The increase in inhibitor concentration and immersion time showed a positive effect on inhibition efficiency. EIS exhibited one capacitive loop which indicates that the corrosion reaction is controlled by charge transfer process. The increase of phase shift (n) in presence of (ACLE) lower surface roughness. This change reveals the adsorption of the inhibitor compound on the steel surface. According to the results of weight loss measurements, the adsorption of the extract on the steel surface can be described by the Langmuir isotherm. The inhibition mechanism of (ACLE) molecules involves physical interaction between the inhibitor and metal surface. Additionally, Protective film formation against acid attack was confirmed by FT-IR and AFM techniques.
Several species of Solanum produce secondary metabolites with antimicrobial activity. In the present study, the inhibitory activity of Solanum chrysotrichum, S. erianthum, S. torvum and S. rostratum against phytopathogenic Curvularia lunata was determined. Methanol extracts from roots, stems, leaves and fruits were evaluated by the method of mycelial inhibition on agar and the minimum inhibitory concentration (MIC) was determined on a liquid medium. To increase the antimicrobial activity, the combined activity of the most active extracts for each phytopathogen was also determined (a combination of intra and interspecies extracts). The results showed that 12 of the 16 methanolic extracts of Solanum species had antifungal effects against C. lunata. The extracts of S. rostratum and S. erianthum developed the highest activity (~80% inhibition and 28.4 MIC μg . ml–1), even, equal to or greater than, the reference fungicide. The mixture of the active extracts of S. chrysotrichum and S. torvum increased their activity. Various extracts affected the macro and microscopic morphology and most of them reduced the number of conidia of the fungus. This resulted in the capacity to control the vegetative growth and reproduction of C. lunata, the causal fungus of corn leaf spot disease.