Details

Title

Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriae in fecal samples from pigs

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2012

Issue

No 2

Authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Publisher

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Date

2012

Identifier

DOI: 10.2478/v10181-011-0144-2 ; ISSN 1505-1773

Source

Polish Journal of Veterinary Sciences; 2012; No 2

References

Akase S. (2009), Application of real time PCR for diagnosis of Swine Dysentery, J Vet Med Sci, 71, 359, doi.org/10.1292/jvms.71.359 ; Elder R. (1994), Rapid detection of <i>Serpulina hyodysenteriae</i> in diagnostic specimens by PCR, J Clin Microbiol, 32, 1497. ; Freschi C. (2005), Comparison of DNA-extraction methods and selective enrichment broths on the detection of <i>Salmonella typhimurium</i> in swine feces by polymerase chain reaction (PCR), Braz J Microbiol, 36, 363, doi.org/10.1590/S1517-83822005000400011 ; Hampson D. (2006), Diseases of Swine, 785. ; Jacobson M. (2004), Routine diagnostics of <i>Lawsonia intracellularis</i> performed by PCR, serological and post mortem examination, with special emphasis on sample preparation methods for PCR, Vet Microbiol, 102, 189, doi.org/10.1016/j.vetmic.2004.04.014 ; Jensen T. (1997), Comparison of histology, immunohistochemistry and polymerase chain reaction for detection of <i>Lawsonia intracellularis</i> in natural porcine proliferative enteropathy, Eur J Vet Pathol, 3, 115. ; Kunkle R. (1988), Improved selective medium for the isolation of Treponema hyodysenteriae, J Clin Microbiol, 26, 2357. ; Lindecrona R. (2002), Application of a 5' nuclease assay for detection of <i>Lawsonia intracellularis</i> in fecal samples from pigs, J Clin Microbiol, 40, 984, doi.org/10.1128/JCM.40.3.984-987.2002 ; McOrist S. (2006), Diseases of Swine, 727. ; Nathues H. (2009), Quantification of <i>Lawsonia intracellularis</i> in porcine faeces by real-time PCR, J Appl Microbiol, 107, 2009, doi.org/10.1111/j.1365-2672.2009.04389.x ; Neef N. (1994), Pathogenicity of porcine intestinal spirochetes in gnobiotic pigs, Infect Immun, 62, 2395. ; Nelson E. (2010), Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, Formatex, 1, 1479. ; Osek J. (2001), Multiplex polymerase chain reaction assay for identification of enterotoxigenic Escherichia coli strains, J Vet Diagn Invest, 13, 308, doi.org/10.1177/104063870101300405 ; Smith S. (1997), Development of persistent intestinal infection and excretion of <i>Lawsonia intracellularis</i> by piglets, Res Vet Sci, 62, 6, doi.org/10.1016/S0034-5288(97)90171-5 ; Song Y. (2004), Real-time PCR quantitation of clostridia in feces of autistic children, Appl Environ Microbiol, 70, 6459, doi.org/10.1128/AEM.70.11.6459-6465.2004 ; Szynkiewicz Z. (1986), Evaluation of selective media for primary isolation of <i>Treponema hyodysenteriae</i> and Treponema innocens, Comp Immunol Microbiol Infect Dis, 9, 71, doi.org/10.1016/0147-9571(86)90077-9 ; Yeh J. (2006), Isolation of <i>Lawsonia intracellularis</i> in Korea and reproduction of proliferative enteropathy in pigs and hamsters, J Vet Med Sci, 68, 499, doi.org/10.1292/jvms.68.499 ; Żmudzki J. (2004), Rapid detection of <i>Brachyspira hyodysenteriae</i> and <i>Lawsonia intracellularis</i> in swine faecal and mucosal specimens by multiplex PCR, Bull Vet Inst Pulawy, 48, 207.
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