TY - JOUR N2 - Biosynthesis of lovastatin (a polyketide metabolite of Aspergillus terreus) in bioreactors of different working volume was studied to indicate how the change of scale of the process influences the formation of this metabolite. The experiments conducted in shake flasks of 150 ml working volume allowed to obtain lovastatin titres at the level of 87.5 mg LOV l-1, when two carbon sources, namely lactose and glycerol were used. The application of the same components in a large stirred-tank bioreactor of 5.3-litre working volume caused a decrease of lovastatin production by 87% compared to the shake flask culture. The deficiency of nitrogen in this bioreactor did not favour the formation of lovastatin, in contrast to the small bioreactor of 1.95-litre working volume, in which lovastatin titres comparable to those in the shake flasks could be achieved, when organic nitrogen concentration was two-fold decreased. When the control of pH and/or pO2 was used simultaneously, an increase in lovastatin production was observed in the bioreactors. However, these results were still slightly lower than lovastatin titres obtained in the shake flasks. L1 - http://www.czasopisma.pan.pl/Content/84682/PDF/07-ZS-paper-Pawlak.pdf L2 - http://www.czasopisma.pan.pl/Content/84682 PY - 2012 IS - No 1 March EP - 84 DO - 10.2478/v10176-012-0007-0 KW - lovastatin KW - Aspergillus terreus ATCC 20542 KW - shake flask KW - stirred tank bioreactor KW - batch process A1 - Pawlak, Marta A1 - Ledakowicz, Stanisław A1 - Bizukojć, Marcin PB - Polish Academy of Sciences Committee of Chemical and Process Engineering DA - 2012 T1 - Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture SP - 71 UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/84682 T2 - Chemical and Process Engineering ER -