TY - JOUR N2 - An effective procedure for producing transformed Centaurium erythraea plants from synthetic seeds is described. Explants were encapsulated in 3% sodium alginate with 3% sucrose. Encapsulated hairy roots were cultured on half-strength Murashige and Skoog (1/2 MS) or Woody Plant (WPM) agar-solidified regeneration media supplemented with 6-benzylaminopurine (BAP) or without the cytokinin. The use of WPM with 0.5 mg/L of BAP gave the best shoot formation frequency (86%) and mean number of shoots (15) per root segment. Shoots rooted with 97% frequency on 1/2 MS without growth regulators. Encapsulated shoot buds were cultured on onethird- strength MS agar medium (1/3 MS) supplemented with indole-3-butyric acid (IBA) (0.05 mg/L). The plantlet conversion frequency was 32%. The encapsulated hairy roots and shoot buds were stored for 4, 6 or 14 weeks at 4°C. Synthetic seeds encapsulated with 3% sodium alginate with 3% sucrose stored at 4°C remained viable for 6 weeks but their developmental parameters significantly decreased. Adding nutrient medium and growth regulator to the alginate matrix increased plantlet recovery from both non-stored and stored synthetic seeds: synthetic seeds retained their viability and ability to form plantlets even after 14 weeks of storage. Regenerated transformed plantlets of C. erythraea were acclimatized in the greenhouse. L1 - http://www.czasopisma.pan.pl/Content/81797/PDF/04_piatczak.pdf L2 - http://www.czasopisma.pan.pl/Content/81797 PY - 2013 IS - No 2 DO - 10.2478/abcsb-2013-0022 KW - Centaurium erythraea KW - hairy roots KW - transgenic plants KW - synthetic seeds A1 - Piątczak, Ewelina A1 - Wysokińska, Halina PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 55 DA - 2013 T1 - ENCAPSULATION OF CENTAURIUM ERYTHRAEA RAFN – AN EFFICIENT METHOD FOR REGENERATION OF TRANSGENIC PLANTS UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/81797 T2 - Acta Biologica Cracoviensia s. Botanica ER -