TY - JOUR N2 - Using four Polish Vicia faba L. minor cultivars (Bronto, Dino, Tibo, Nadwiślański) we obtained callus from epicotyl fragments collected from 7- and 14-day-old seedlings and from cotyledonary nodes of immature seeds. Callus induction efficiency varied from 81% to 97% depending on the origin of the explant. Shoots regenerated only from the cotyledonary nodes of all tested cultivars. On average, 50% of the explants grown on MS medium containing 1.0 mg dm-3 NAA, 0.5 mg dm-3 BAP, 0.25 mg dm-3 GA3, 1.0 g dm-3 casein hydrolysate, 750 mg dm-3 inositol, 3% sucrose and 0.4% agar were able to regenerate shoots. The number of calluses regenerating shoots was highest from explants collected from fruiting nodes 6 to 9. Decapitation of donor plants increased the percentage of calluses regenerating shoots. On half-strength MS medium with 2 mg dm-3 NAA and on 1/2 MS alone, 11% of the shoots rooted; on 1/2 MS with 1 g dm-3 AC, 8.0% rooted. The regenerants were transferred to Perlite with Hoagland medium and acclimated. Ten percent of the regenerated plants survived the acclimation process, flowered and produced seeds. L1 - http://www.czasopisma.pan.pl/Content/81776/PDF/v10182-012-0026-7.pdf L2 - http://www.czasopisma.pan.pl/Content/81776 PY - 2012 IS - No 2 DO - 10.2478/v10182-012-0026-7 KW - callus KW - immature seed KW - organogenesis KW - in vitro culture KW - Vicia faba L. minor A1 - Czyczyło-Mysza, Ilona A1 - Skrzypek, Edyta A1 - Marcińska, Izabela PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 54 DA - 2012 T1 - Indirect Organogenesis of Faba Bean (Vicia Faba L. Minor) UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/81776 T2 - Acta Biologica Cracoviensia s. Botanica ER -