TY - JOUR N2 - We compared the biochemical profiles of Physalis ixocarpa hairy roots transformed with Agrobacterium rhizogenes ATCC and A4 strains with non-transformed root cultures. The studied clones of A4- and ATCC-induced hairy roots differed significantly; the latter showed greater growth potential and greater ability to produce secondary metabolites (tropane alkaloids) and to biotransform hydroquinone to arbutin. We compared glucose content, alanine and aspartate aminotransferase activity, and L-phenylalanine ammonia-lyase activity. We analyzed markers of prooxidant/antioxidant homeostasis: catalase, ascorbate peroxidase, oxidase, glutathione peroxidase and transferase activity, and the levels of ascorbate, glutathione, tocopherol and lipid peroxidation. We found that transformation induced strain-specific regulation, including regulation based on redox signals, determining the rate of allocation of carbon and nitrogen resources to secondary metabolism pathways. Our results provide evidence that A. rhizogenes strain-specific modification of primary metabolites contributed to regulation of secondary metabolism and could determine the ability of P. ixocarpa hairy root clones to produce tropane alkaloids and to convert exogenously applied hydroquinone to pharmaceutically valuable arbutin. Of the studied parameters, glucose content, L-phenylalanine ammonia-lyase activity and alanine aminotransferases activity may be indicators of the secondary metabolite-producing potential of different P. ixocarpa hairy root clones. L1 - http://www.czasopisma.pan.pl/Content/81750/PDF/03_bergier.pdf L2 - http://www.czasopisma.pan.pl/Content/81750 PY - 2012 IS - No 1 DO - 10.2478/v10182-012-0002-2 KW - Agrobacterium rhizogenes KW - arbutin KW - hairy roots KW - Physalis KW - primary and secondary metabolism KW - tropane alkaloids A1 - Bergier, Katarzyna A1 - Kuźniak, Elżbieta A1 - Wielanek, Marzena A1 - Królicka, Aleksandra A1 - Skłodowska, Maria PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 54 DA - 2012 T1 - Antioxidant Profiles and Selected Parameters of Primary Metabolism in Physalis Ixocarpa Hairy Roots transformed by two Agrobacterium Rhizogenes Strains UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/81750 T2 - Acta Biologica Cracoviensia s. Botanica ER -