TY - JOUR N2 - To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies, but a system in which SE or ORG can be induced alternatively in the same type of explant has not been proposed. Immature zygotic embryos (IZEs) of Arabidopsis provide the only explants with embryogenic potential, and have been recommended for studying mechanisms of SE induced in vitro. This study was aimed at defining culture conditions promoting induction of alternative morphogenic pathways: shoot ORG in IZE explants. The established protocol involves pretreatment of IZE explants with liquid auxin-rich callus induction (CIM) medium, followed by subculture on solid cytokinin-rich shoot induction medium (SIM). The method enables efficient shoot induction in Columbia (Col-0) and Wassilewskija (Ws), genotypes commonly used in molecular studies. During 3 weeks of culture up to 90% of Col-0 and 70% of Ws explants regenerated shoots via an indirect morphogenic pathway. We analyzed the qRT-PCR expression patterns of the LEC (LEC1, LEC2 and FUS3) genes, the key regulators of Arabidopsis embryogenesis, in the IZE explants induced to promote shoot ORG. The sharp decline of LEC expression on SIM medium confirmed that culture of Arabidopsis IZE explants enables experimental manipulation of the morphogenic response of somatic cells. A scheme illustrating various in vitro morphogenic responses of IZEs in relation to hormonal treatment is presented. L1 - http://www.czasopisma.pan.pl/Content/81738/PDF/abc_53_2_07.pdf L2 - http://www.czasopisma.pan.pl/Content/81738 PY - 2011 IS - No 2 DO - 10.2478/v10182-011-0028-x KW - Arabidopsis KW - immature zygotic embryos KW - in vitro morphogenesis KW - shoot regeneration A1 - Kraut, Małgorzata A1 - Wójcikowska, Barbara A1 - Ledwoń, Agnieszka A1 - Gaj, Małgorzata PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 53 DA - 2011 T1 - Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/81738 T2 - Acta Biologica Cracoviensia s. Botanica ER -