TY - JOUR N2 - Emerging researches in humans, pigs and mice, highlighted that estrogen plays a pivotal role in self-renewal and differentiation of bone marrow mesenchymal stem cells (BMSCs). The present study aimed at evaluating effects of 17 beta-estradiol (E2) on proliferation and apoptosis of canine-derived bone marrow mesenchymal stem cells (cBMSCs) in vitro. The results showed that E2 supplementation at the concentration of 10-11 M promoted the proliferation of cBMSCs by CCK-8 assay and RT-qPCR analysis for the proliferation-related genes, with proliferating cell nuclear antigen (PCNA), cyclin-D1 (CCND1) being up-regulated and cyclin-dependent kinase inhibitor 1B (CDKN1B) being down-regulated. Contrarily, analysis of fluorescence-activated cell sorting (FACS) and RT-qPCR demonstrated that E2 supplementation above 10-11 M had inhibitory effects on the proliferation of cBMSCs and induced apoptosis. Intriguingly, cBMSCs still possessed the capability to differentiate into osteoblasts and adipocytes with 10-11 M E2 addition. Taken together, this study determined the optimal culture condition of cBMSCs in vitro, and has important implications for further understanding the regulatory effect of E2 on the self-renewal of cBMSCs, which are helpful for the clinical application of BMSCs. L1 - http://www.czasopisma.pan.pl/Content/116768/PDF/10.pdf L2 - http://www.czasopisma.pan.pl/Content/116768 PY - 2020 IS - No 2 EP - 245 DO - 10.24425/pjvs.2020.133638 KW - apoptosis KW - bone marrow mesenchymal stem cells (BMSCs) KW - canine KW - proliferation KW - 17 beta-estradiol (E2) A1 - Zhou, Z.-H. A1 - Gu, C.-W. A1 - Li, J. A1 - Huang, X.-Y. A1 - Deng, J.-Q. A1 - Shen, L.-H. A1 - Cao, S.-Z. A1 - Deng, J.-L. A1 - Zuo, Z.-C. A1 - Wang, Y. A1 - Ma, X.-P. A1 - Ren, Z.-H. A1 - Yu, S.-M. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 23 DA - 2020.06.29 T1 - 17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro SP - 235 UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/116768 T2 - Polish Journal of Veterinary Sciences ER -