TY - JOUR N2 - Effect of single nucleotide polymorphism (SNP) in splicing site of the LPAR1 (lysophosphatidic acid receptor 1) gene on selected quality traits was investigated in frozen-thawed semen of Holstein-Friesian bulls. Splicing mutation A/G in the LPAR1 gene (rs43581860) was identified in 120 Holstein-Friesian bulls using PCR-RFLP technique (Hph I). Heterozygotes AG were the most frequent (37.5%) compared with AA (30.8%) and GG (31.7%) homozygotes. Observed differences in total motility (TM), sperm membrane integrity (SYBR-14/PI) and ATP content were significant between homozygotes AA or GG and heterozygotes AG. For all three traits disadvantageous effect of heterozygotes AG was detected. This means that LPAR1 splicing mutation has significant effect on semen quality and should be considered as a new marker of semen quality in Holstein-Friesian bulls. L1 - http://www.czasopisma.pan.pl/Content/106883/PDF/25.pdf L2 - http://www.czasopisma.pan.pl/Content/106883 PY - 2018 IS - No 2 EP - 421 DO - 10.24425/122611 KW - bull KW - sperm KW - quality KW - LPAR1 KW - polymorphism A1 - KamiƄski, S. A1 - Hering, D.M. A1 - Kordan, W. A1 - Lecewicz, M. A1 - Sazanov, A. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 21 DA - 2018.08.30 T1 - Influence of splicing mutation within the lysophosphatidic acid receptor 1 gene (LPAR1) on semen quality in Holstein-Friesian bulls SP - 419 UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/106883 T2 - Polish Journal of Veterinary Sciences ER -