TY - JOUR N2 - An efficient system of micropropagation via somatic embryogenesis from root-derived callus was established in Arabica coffee (Coffea arabica L.). Twenty-six callus lines were induced on MS (Murashige and Skoog, 1962) medium supplemented with combinations of NAA (0, 0.1, 0.5, 1 and 2 mg/L) plus BA (0, 1 and 2 mg/L), or 2,4-D (0, 0.1, 0.5, 1 and 2 mg/L) plus TDZ (0, 1 and 2 mg/L). Subsequently, two types of somatic embryos were obtained from callus cultures and named S-type and I-type embryos. The S-type embryos were obtained from an 18-monthold callus line which was induced and maintained at 2 mg/L TDZ and 0.1 mg/L 2,4-D near the end of each period of the subculture. These embryos have a developmental barrier, which did not pass through the torpedo stage and could be overcome by a supplement of 2 or 5 mg/L BA. The I-type embryos were induced from 3-month-old callus when transferred onto induction media, i.e., MS supplemented with TDZ (2 and 5 mg/L) plus 2,4-D (0 and 0.1 mg/L). The significantly highest response, i.e., 13.3 embryos per callus clump was obtained at 2 mg/L TDZ. In this study, the results reveal that TDZ has a crucial effect on embryogenic callus induction, proliferation and subsequent somatic embryogenesis. L1 - http://www.czasopisma.pan.pl/Content/102670/PDF/ABC%20Bot.%202-18%204-Y.Wang%20i%20in.pdf L2 - http://www.czasopisma.pan.pl/Content/102670 PY - 2018 IS - No 2 EP - 35–44 DO - 10.24425/118053 KW - callus KW - developmental barrier KW - embryogenic capacity KW - somatic embryo A1 - Wang, Yi-Chieh A1 - Lin, Meng-Ze A1 - Huang, Bin A1 - Chung, Hsiao-Hang A1 - Chen, Jen-Tsung PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 60 DA - 2018.09.26 T1 - Thidiazuron Enhanced Somatic Embryogenes is from Callus Lines of Arabica Coffee and Subsequent Plant Regeneration SP - 35–44 UR - http://www.czasopisma.pan.pl/dlibra/publication/edition/102670 T2 - Acta Biologica Cracoviensia s. Botanica ER -