@ARTICLE{Ting_Shan_Yao_Qa-SNARE_2015, author={Ting Shan Yao and Xiao Feng Zhu and Jin Hee Jung and Yuan Hu Xuan}, volume={vol. 57}, number={No 2}, journal={Acta Biologica Cracoviensia s. Botanica}, howpublished={online}, year={2015}, publisher={Biological Commission of the Polish Academy of Sciences – Cracow Branch}, abstract={Abstract Soluble N-ethyl-maleimide sensitive factor attachment adaptor protein receptor (SNARE) domain-containing proteins were mainly involved in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNARE in different aspects of plant biology. Among them, Synthaxin-22 (SYP22) a Qa-SNARE has been reported to have a pleiotropic function in plant development including regulation of leaf waving, shoot gravitropism and flowering time. In this study, we identified a new role of SYP22 in regulation of brassinosteroid (BR) signaling, especially in the dark. SYP22 interacts with BR receptor, brassinosteroid insensitive 1 (BRI1), and overexpression of SYP22 enhanced a weak BRI1 mutant bri1-5 phenotype. syp22 mutant exhibits short hypocotyl and it is sensitive to exogenously treated BR while slightly insensitive to BR-biosynthesis inhibitor propiconazole (PCZ) in the dark. Expression levels of BR signaling maker genes ACS5, SAUR15 and IAA19 were slightly higher, while BR6OX2, a BR biosynthesis marker gene, was lower in syp22 compared to the wild-type. In addition, syp22 was sensitive to 2,4-D, a synthetic auxin, in the dark. In conclusion, SYP22 is involved in BR- and auxin-mediated hypocotyl growth inhibition in the dark, which might be via interaction with BR and auxin key regulators to alter their internalization in Arabidopsis.}, title={Qa-SNARE Protein SYP22 Negatively Regulates Brassinosteroid Signaling in the Dark}, URL={http://www.czasopisma.pan.pl/Content/81862/PDF/07_yao.pdf}, doi={10.1515/abcsb-2015-0021}, }