@ARTICLE{Yu_S._Polymorphic_2019, author={Yu, S. and Wu, J. and Bai, J. and Ding, Y. and Qiu, W. and Zhang, L.}, volume={vol.22}, number={No 2}, journal={Polish Journal of Veterinary Sciences}, pages={415-422}, howpublished={online}, year={2019}, publisher={Polish Academy of Sciences Committee of Veterinary Sciences}, publisher={University of Warmia and Mazury in Olsztyn}, abstract={Function of duck (Anas platyrhynchos) major histocompatibility complex class I (Anpl-MHC I) molecules in binding peptides is through the peptide binding groove (PBG), which is thought to be influenced by the high polymorphism of α1 and α2 domains. However, little is known about the polymorphism of Anpl-MHC I peptide binding domain (PBD), especially in the domestic duck. Here, we analyzed the polymorphism of forty-eight Anpl-MHC I α1 and α2 domains from domestic duck breeds previously reported. All sequences were analyzed through multiple sequence alignment and a phylogenetic tree was constructed. The coefficient of variance of the peptide binding domains (PBDs) from WS, CV, JD, and SX duck breeds was estimated based on the Wu-Kabat variability index, followed by the location of the highly variable sites (HVSs) on reported crystal structure models. Analysis of α1 and α2 domains showed common features of classical MHC class I and high polymorphism, especially in α1 domain. The constructed phylogenetic tree showed that PBDs of domestic ducks did not segregate based on breeds and had a close phylogenetic relationship, even with wild ducks. In each breed, HVSs were mostly located in the PBG, suggesting that they might determine peptide-binding characteristics and subsequently influence peptide presentation and recognition. The combined results of sequence data and crystal structure provide novel valuable insights into the polymorphism and diversity of Anpl-MHC I PBDs that will facilitate further studies on disease resistance differences between duck breeds and the development of cytotoxic T-lymphocyte (CTL) epitope vaccines suited for preventing diseases in domestic ducks.}, type={Article}, title={Polymorphic analysis of peptide binding domain of major histocompatibility complex class I in domestic ducks}, URL={http://www.czasopisma.pan.pl/Content/112848/PDF/28.pdf}, doi={10.24425/pjvs.2019.129302}, keywords={domestic duck, highly variably sites, MHC class I, peptide binding domain, phylogenetic tree, polymorphism}, }