@ARTICLE{Ismail_Manal_Sayed_Mohamed_Acaricidal,_2019,
 author={Ismail, Manal Sayed Mohamed and Tag, Hend Maroof and Rizk, Marguerite Adly},
 volume={vol. 59},
 number={No 2},
 journal={Journal of Plant Protection Research},
 pages={151-159},
 howpublished={online},
 year={2019},
 publisher={Committee of Plant Protection PAS},
 publisher={Institute of Plant Protection – National Research Institute},
 abstract={This study investigates the acaricidal, ovicidal, and repellent effects of the Tagetes patula Linn. (Asteraceae) leaf extract against both the adult female and egg stages of Tetranychus urticae Koch (Trombidiformes: Tetranychidae) under laboratory conditions. The Tagetes patula ethanolic leaf extract [TpEtOH70%] was screened for adulticide and ovicide bioassays in order to consider its acute toxicity. One sublethal concentration was used to assess egg-laying capacity (fecundity), repellent, and oviposition deterrent activities. The chemical characterization was conducted by gas chromatography-mass spectrometry (GC-MS) analysis to identify the TpEtOH70% bioactive components. Results showed that the LC50 value of TpEtOH70% leaf extract predicted by Probit analysis against T. urticae adult females at 24 h was 0.99%. The TpEtOH70% leaf extract showed a significant toxic effect as the highest mean mortality rates (± SE) of the treated adult females was 88.9 ± 3.7%. However, the TpEtOH70% leaf extract was insignificant in affecting the egg-laying capacity of the adult females treated with a sublethal dose of 0.5% even after 72 h. The TpEtOH70% leaf extract was classified repellent since the repellent index (RI) value was lower than 1 – SD. In addition, it had a high oviposition deterring effect based on a 100% reduction of the total number of eggs. The TpEtOH70% leaf extract had a significant ovicidal effect on T. urticae eggs, with 56.04% reduction in hatching. Five bioactive compounds from various classes of phytochemicals were identified in the TpEtOH70% leaf extract and the major compound was phytol (62.72%). This pioneering investigation reveals the adulticidal, ovicidal, and repellent activities of the TpEtOH70% leaf extract against T. urticae. A combination of multiple modes of action of different plant components may act alone or in synergism to delay the development of mite resistance.},
 type={Artykuły / Articles},
 title={Acaricidal, ovicidal, and repellent effects of Tagetes patula leaf extract against Tetranychus urticae Koch (Acari: Tetranychidae)},
 URL={http://www.czasopisma.pan.pl/Content/112832/PDF/AO2_JPPR_59_2_236_Ismail.pdf},
 doi={10.24425/jppr.2019.129285},
 keywords={acaricide, GC-MS analysis, leaf extract, repellent, Tetranychus urticae, Tagetes patula},
}