@ARTICLE{Wu_J._pUC18-CpG_2019,
 author={Wu, J. and Chen, Q. and Xin, T. and Sun, Y. and Jia, H. and Hou, S.H. and Guo, X.Y.},
 volume={vol.22},
 number={No 2},
 journal={Polish Journal of Veterinary Sciences},
 pages={195–201},
 howpublished={online},
 year={2019},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-β, and pro-inflammatory cytokines TNF-α and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adju- vanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response.},
 type={Article},
 title={pUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice},
 URL={http://www.czasopisma.pan.pl/Content/110886/PDF/01.pdf},
 doi={10.24425/pjvs.2019.127086},
 keywords={adjuvant, CpG DNA, Echinococcus granulosus, TBK1, type I interferons},
}