@ARTICLE{Fraser_L._Total_2019, author={Fraser, L. and Brym, P. and Mogielnicka-Brzozowska, M. and Wasilewska, K.}, volume={vol.22}, number={No 1}, journal={Polish Journal of Veterinary Sciences}, pages={181-185}, howpublished={online}, year={2019}, publisher={Polish Academy of Sciences Committee of Veterinary Sciences}, publisher={University of Warmia and Mazury in Olsztyn}, abstract={In this study the quality of total RNA, isolated from fresh spermatozoa, was compared between boars with good and poor semen freezability (GSF and PSF, respectively). Semen from 3 boars with GSF exhibited significantly higher total motility, mitochondrial function, plasma membrane integrity and reduced lipid peroxidation compared with 3 boars with PSF after cryo- preservation. There were variations in the quality of RNA isolated from spermatozoa of boars with GSF and PSF. Boars with GSF exhibited mainly full-length, intact RNA, whereas substantial amounts of degraded RNA were detected in spermatozoa from boars with PSF. Further under- standing of the biological relevance of RNAs in sperm function is critical to improve the freezabil- ity of boar semen.}, type={Short communication}, title={Total RNA quality in boar spermatozoa with different freezability}, URL={http://www.czasopisma.pan.pl/Content/110885/PDF/25.pdf}, doi={10.24425/pjvs.2019.127085}, keywords={boar, spermatozoa, semen freezability, RNA profiles}, }