@ARTICLE{Kamiński_S._Influence_2018,
 author={Kamiński, S. and Hering, D.M. and Kordan, W. and Lecewicz, M. and Sazanov, A.},
 volume={vol. 21},
 number={No 2},
 journal={Polish Journal of Veterinary Sciences},
 pages={419-421},
 howpublished={online},
 year={2018},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={Effect of single nucleotide polymorphism (SNP) in splicing site of the LPAR1 (lysophosphatidic acid receptor 1) gene on selected quality traits was investigated in frozen-thawed semen of Holstein-Friesian bulls. Splicing mutation A/G in the LPAR1 gene (rs43581860) was identified in 120 Holstein-Friesian bulls using PCR-RFLP technique (Hph I). Heterozygotes AG were the most frequent (37.5%) compared with AA (30.8%) and GG (31.7%) homozygotes. Observed differences in total motility (TM), sperm membrane integrity (SYBR-14/PI) and ATP content were significant between homozygotes AA or GG and heterozygotes AG. For all three traits disadvantageous effect of heterozygotes AG was detected. This means that LPAR1 splicing mutation has significant effect on semen quality and should be considered as a new marker of semen quality in Holstein-Friesian bulls.},
 type={Artykuły / Articles},
 title={Influence of splicing mutation within the lysophosphatidic acid receptor 1 gene (LPAR1) on semen quality in Holstein-Friesian bulls},
 URL={http://www.czasopisma.pan.pl/Content/106883/PDF/25.pdf},
 doi={10.24425/122611},
 keywords={bull, sperm, quality, LPAR1, polymorphism},
}